Antimicrobial evaluation of crude extracts of bark and stem of virginia creeper (Parthenocissus quinquefolia (L.) Planch)
DOI:
https://doi.org/10.55627/zoobotanica.001.02.0608Keywords:
Antimicrobial activity, E. coli, inhibition zone, minimum inhibitory concentration, virginia creeperAbstract
The In vitro antimicrobial potential of bark and stem of virginia creeper (Parthenocissus quinquefolia (L.) Planch) was measured in order to authenticate its ethnopharmacological significance. Bark and stem parts were extracted by static maceration technique in non-polar and polar solvents, such as n-hexane, chloroform, ethanol and double distilled water. Agar well diffusion technique was used to verify the antimicrobial activity of these extracts against two of each Gram-negative, Gram-positive and fungal strains. Minimum inhibitory concentration (MIC) was measured through modified broth dilution method. However, ethanolic and aqueous extracts of stem resulted in highest inhibition zone (52.5±1.1 mm) against Pseudomonas aeruginosa, and augmented antifungal activity (46±0.48 mm) against Fusarium solani, respectively. Whereas, n-hexane extract of bark exhibited least antibacterial activity in the form of inhibition zone of 15±0.22mm against S. aureus. Similarly, minimum activity (16.5±0.21 mm) was observed in ethanolic extract of bark, against F. solani. The most resistant MIC value was shown by ethanolic bark extract, i.e. 0.5 mg/mL against P. aeruginosa. Thus, virginia creeper can be recommended as a remedy to combat efficaciously with the resilient contagious strains.
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