Comparative Study of Natural (Lactic Acid) and Synthetic (Pyodine) Antiseptic Teat Dips for Mastitis Control in Dairy Cattle

Authors

  • Waqas Ahmad Department of Livestock Management, Breeding & Genetics, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar
  • Iftikhar Ahmad Department of Livestock Management, Breeding & Genetics, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar
  • Muhammad shuaib Arid Zone Small Ruminants Research Institute Ghulam Banda Kohat
  • Obaid Ullah Faculty of Veterinary Sciences, The University of Veterinary and Animal Sciences Swat
  • Sohaib Ul Hassan College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar
  • Danyal Khan Department of Livestock Management, Breeding & Genetics, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar
  • Muhammad Shahkar Uzair Department of Livestock Production and Management, Faculty of Veterinary Sciences, The University of Veterinary and Animal Sciences Swat
  • Abdul Jabbar Faculty of Veterinary and Animal Sciences, University of Poonch Rawalakot
  • Muhammad Idrees Arid Zone Small Ruminants Research Institute, Ghulam Banda, Kohat

DOI:

https://doi.org/10.55627/agrivet.005.01.1761

Keywords:

Bovine mastitis, Treat dipping, Lactic acid, Pyodine, Dairy cattle, Antiseptic

Abstract

Bovine mastitis is one of the most economically important diseases affecting dairy cattle worldwide, leading to reduced milk yield, altered milk composition, and increased treatment costs. The present study aimed to evaluate the effectiveness of natural and synthetic antiseptic solutions used for post-milking teat dipping to control subclinical mastitis in dairy cows and to assess their economic feasibility. A total of 20 lactating dairy cows with healthy udders were selected and randomly divided into five groups (n = 4 cows per group). Group G-1 served as the control, while G-2 and G-3 received lactic acid teat dip solutions at concentrations of 2% and 5%, respectively. Groups G-4 and G-5 received synthetic antiseptic pyodine solutions at concentrations of 2% and 5%, respectively. The experiment lasted 28 days. Milk samples were collected weekly and screened for subclinical mastitis using the California Mastitis Test (CMT) and Surf Field Mastitis Test (SFMT). The results indicated that the highest incidence of mastitis occurred in the control group (G-1), followed by G-4, G-2, and G-5, whereas no cases of subclinical mastitis were recorded in the G-3 group during the entire experimental period. The economic evaluation showed that pyodine-based solutions were less expensive than lactic acid solutions. However, the 5% lactic acid treatment demonstrated superior efficacy in reducing subclinical mastitis. The findings suggest that lactic acid teat dipping can serve as an effective natural alternative for mastitis control in dairy cattle, although further studies with larger sample sizes are recommended.

References

Abdalla, AM., El-Sayed, A., Awad, A. 2023. Recent advances in bovine mastitis control strategies in dairy cattle. Animals. 13:1452.

Breen, J., 2019. The importance of teat disinfection in mastitis control. Livestock, 24(3), 122-128.

Costa, J. C. M., Espeschit, I. D. F., Pieri, F. A., Benjamin, L. A., & Moreira, M. A. S., 2014. Increase in biofilm formation by Escherichia coli under conditions that mimic the mastitic mammary gland. Ciência Rural, 44, 666-671.

Garcia, M., Sharma, N., Singh, V. 2022. Mastitis control programs and teat disinfectants in modern dairy farming. Journal of Dairy Science. 105:8950–8963.

Haider, A., Ikram, M., Shahzadi, I., & Asif., RM., 2023. Bovine mastitis. In Polymeric Nanoparticles for Bovine Mastitis Treatment, 19: 49-80. Cham: Springer Nature Switzerland.

Isaac, P., Kumar, A., Sharma, N. 2017. Antibacterial and biofilm inhibition activity of teat disinfectant compounds against mastitis pathogens. Veterinary Microbiology. 203:66–72.

Koti, K., Rodas-Gonzalez, A., Nadon, C., McAllister, T., Yang, X., and Narváez-Bravo, C., 2024. Evaluating disinfectant efficacy on mixed biofilms comprising Shiga toxigenic Escherichia coli, lactic acid bacteria, and spoilage microorganisms. Frontiers in Microbiology, 15, 1360645.

Kunwar, H., 2024. Lactic acid bacteria with antagonistic properties towards dairy food microbial contaminants and mastitis pathogens (Doctoral dissertation, Swinburne).

Nguyen, TT., Lee, SH., Kim, HJ. 2024. Natural antimicrobial agents for controlling bovine mastitis pathogens. Veterinary Microbiology. 285:109906.

Oliver, SP., Murinda, SE., Jayarao, BM. 2012. Impact of teat dipping on prevention of bovine mastitis. Veterinary Clinics of North America: Food Animal Practice. 28:217–229.

Patel, J., Patel, R. 2022. Role of organic acids in controlling bacterial infections in dairy animals. Veterinary World. 15:2458–2466.

Rahman, M., Islam, M., Hossain, M. 2023. Evaluation of post-milking teat disinfectants for the control of subclinical mastitis in dairy cattle. Frontiers in Veterinary Science. 10:118420.

Rezaei, M., Shahbazi, Y., Fathi, B. 2021. Effect of iodine teat disinfectants on iodine concentration in milk. Journal of Dairy Research. 88:141–146.

Schmidt, A. L., Oliver, S. P., and Fydenkevez, M. E., 1984. Evaluation of experimental teat dip containing sodium chlorite and lactic acid by excised teat assay. Journal of Dairy Science., 67(12): 3075-3080.

Zhang, L., Wang, Y., Li, H. 2021. Effectiveness of barrier teat dips in preventing subclinical mastitis in dairy cows. Journal of Dairy Science. 104:5678–5686.

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Published

2026-03-30

How to Cite

Comparative Study of Natural (Lactic Acid) and Synthetic (Pyodine) Antiseptic Teat Dips for Mastitis Control in Dairy Cattle (W. Ahmad, I. Ahmad, M. shuaib, O. Ullah, S. U. Hassan, D. Khan, M. S. Uzair, A. Jabbar, & M. Idrees, Trans.). (2026). Journal of Agriculture and Veterinary Science, 5(1), 91-96. https://doi.org/10.55627/agrivet.005.01.1761