Harnessing Soybean Diversity: Evaluating Introduced Genotypes for Sustainable Yield in Pakistan

Authors

  • Zulqar Nain Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara
  • Zaheer Ahmed Center for Advanced Studies in Agriculture and Food Security, University of Agriculture Faisalabad
  • Ahmed Raza Agriculture Testing Lab, Punjab Agriculture Food and Drug Authority, Punjab Science Enclave, Multan Road, Lahore
  • Maqsood Ali Department of Plant Pathology, University of Agriculture Faisalabad, Constituent College, Depalpur Okara
  • Uzair Ahmad Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara
  • Faiz Ullah Arif Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara
  • Muhammad Nasir Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara
  • Muhammad Qasim Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara
  • Muhammad Rizwan Shafiq Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara
  • Sidra Iqbal Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Constituent College, Depalpur, Okara

DOI:

https://doi.org/10.55627/agrivet.005.01.01854

Keywords:

Soybean, Genotypic Variation, Yield Traits, Principal Component Analysis, Cluster Analysis

Abstract

Food security has become a challenging issue in the altered climate pattern, especially for developing countries. Soybean (Glycine max L.), a crop having high protein and edible oil content, has great potential but remains underutilized in Pakistan due to the lack of diverse, locally adapted genotypes. This study aimed to assess the genetic potential of fifteen exotic soybean genotypes, including one check cultivar, Faisal Soybean, by investigating various yield-related traits under the agroclimatic conditions of Okara, Pakistan. This experiment was conducted under a Randomized Complete Block Design (RCBD) with three replications. Analysis of variance revealed highly significant (p < 0.01) genetic variation for plant height (MS = 93.58), number of pods per plant (MS = 152.46), and number of seeds per pod (MS = 1.407). Principal component analysis (PCA) showed that the first two components accounted for 73.02% of the cumulative variability in quantitative traits and 86.12% in qualitative traits, with plant height and pods per plant identified as key yield-related contributing traits. Positive correlations were observed among several traits, such as plant height, primary branches (r = 0.64), pods per plant (r = 0.41), and seeds per pod (r = 0.62). Cluster analysis grouped the genotypes into distinct clusters, with genotypes G1, G10, and G11 showing unique characteristic profiles, while G4, G5, G13, and G15 formed a closely related group. These findings highlight considerable genotypic variation in major traits, contributing to the development of high-yielding, climate-resilient varieties adapted to local conditions.

References

Analytical Software. (2005). Statistix 8.1 user’s manual. Analytical Software.

Al-Hadi, G., R.M. Islam, A.M. Karim and T.M. Islam. 2017. Morpho-physiological characterization of soybean genotypes under subtropical environment. Genetika 49:297-311.

Akram, Z. and Q. Ahmad. 2019. Future prospects of soybean in Pakistan. Biomed J Sci Tech Res 15:11562-11563.

Asad, S.A., M.A. Wahid, S. Farina, R. Ali and F. Muhammad. 2020. Soybean production in Pakistan: experiences, challenges and prospects. International Journal of Agriculture and Biology 24:995-1005.

Amol, V., Bhati, K. R., & Bhati, K. R. (2021). Nutritive benefits of soybean (Glycine max). Indian J Nutr Diet, 522-33.

Abbas, G. (2023). Effect of Soybean Unavailability Situattions and COVID-19 on the Poultry Industry of Pakistan: A Comprehensive Analysis Problems Faced and Its Solution for Sustainable Animal Production. Pakistan Journal of Science, 75(2).

Dubey, N., A. Shrivasthava, H. Avinashe and S. Jaiwar. 2015. Genetic variability, correlation and path analysis for yield and yield contributing characters in soybean (Glycine max L.). Electronic Journal of Plant Breeding 6:318-325.

Iqbal, Z., M. Arshad, M. Ashraf, T. Mahmood and A. Waheed. 2008. Evaluation of soybean [Glycine max (L.) Merrill] germplasm for some important morphological traits using multivariate analysis. Pakistan Journal of Botany 40:2323-2328.

Johnson, H.W., H. Robinson and R. Comstock. 1955. Estimates of genetic and environmental variability in soybeans.

Jolliffe, I.T. 2002. Principal component analysis for special types of data. Springer.

Jeong, S.-C., J.-K. Moon, S.-K. Park, M.-S. Kim, K. Lee, S.R. Lee, N. Jeong, M.S. Choi, N. Kim and S.-T. Kang. 2019. Genetic diversity patterns and domestication origin of soybean. Theoretical and Applied Genetics 132:1179-1193.

Kumar, A., A. Pandey, C. Aochen and A. Pattanayak. 2015. Evaluation of genetic diversity and interrelationships of agro-morphological characters in soybean (Glycine max) genotypes. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 85:397-405.

Khurshid, H., D. Baig, S. Jan, M. Arshad and M. Khan. 2017. Miracle crop: the present and future of soybean production in Pakistan. MOJ Biology and Medicine 2:189-191.

Mondal, M. and M. Wahhab. 2001. Production Technology of Crops. Oil seed Research Center, Bangladesh Agril. Res. Inst. Joydebpur, Gazipur:1-10.

Moe, S. and T. Girdthai. 2013. Relationships of soybean [Glycine max (L.) Merrill] accessions based on physiological and agro-morphological traits. International Journal of Chemical, Environmental & Biological Sciences (IJCEBS) 1:375-380.

Malek, M., M.Y. Rafii, M. Shahida Sharmin Afroz, U.K. Nath and M.M.A. Mondal. 2014. Morphological characterization and assessment of genetic variability, character association, and divergence in soybean mutants. The Scientific World Journal 2014:968796.

Mahbub, M., M.M. Rahman, M. Hossain, L. Nahar and B. Shirazy. 2016. Morphophysiological variation in soybean (Glycine max (L.) Merrill). American-Eurasian Journal of Agricultural & Environmental Sciences 16:234-238.

Mahbub, M.M. and B.J. Shirazy. 2016. Evaluation of genetic diversity in different genotypes of soybean (Glycine max (L.) Merrill). American Journal of Plant Biology 1:24-29.

Nasir, B., Rahman, S. U., Ali, A., Shafique, E., Zia, N., Ahmad, N., ... & Bukhari, R. (2025). Resilient soybeans for a changing climate: Analyzing traditional and emerging new plant breeding technologies to combat abiotic stresses. Acta Physiologiae Plantarum, 47(11), 102.

Pearson, K. 1895. Contributions to the Mathematical Theory of Evolution. III. Regression, Heredity, and Panmixia. Proceedings of the Royal Society of London 59:69-71.

Pakistan Economy. 2023. Agriculture. Crops 3:3-006.

Suparwata, D. and F. Jamin. 2024. Analysis of Organic Fertilizer Use in Improving Soil Quality and Agricultural Yields in Indonesia. West Science Agro, 2 (01), 17–27.

Singh, K. H., Gupta, S., Shivakumar, M., & Nataraj, V. (2025). Soybean [Glycine max (L.) Merr.] Breeding. In Fundamentals of Legume Breeding: A Text for Students and Practitioners (pp. 283-298). Singapore: Springer Nature Singapore.

Team, R.C. 2024. RA language and environment for statistical computing, R Foundation for Statistical. Computing.

Ullah, A., Akram, Z., Malik, S. I., & Khan, K. S. U. (2021). Assessment of phenotypic and molecular diversity in soybean [Glycine max (L.) Merr.] germplasm using morpho-biochemical attributes and SSR markers. Genetic Resources and Crop Evolution, 68(7), 2827-2847.

Ullah, A., Z. Akram, G. Rasool, M. Waris and H. Khurshid. 2024. Agro-morphological characterization and genetic variability assessment of soybean [Glycine max (L.) Merr.] germplasm for yield and quality traits. Euphytica 220:67.

USDA. 2025 World Agricultural Production. Circular Series WAP. Foreign Agricultural Service, USDA, USA, p. 1-30.

Ward Jr, J.H. 1963. Hierarchical grouping to optimize an objective function. Journal of the American statistical association 58:236-244.

Wang, G., Q. Zhou, M. He, X. Zhong and G. Tang. 2020. Wilting index and root morphological characteristics used as drought-tolerance variety selection at the seedling stage in soybean (Glycine max L.). Plant Growth Regulation 92:29-42.

Zaidi, S.M.H. 2014. Edible oil imports in Pakistan. South Asian Journal of Management 8:1-8.

Downloads

Published

2026-03-30

How to Cite

Harnessing Soybean Diversity: Evaluating Introduced Genotypes for Sustainable Yield in Pakistan (Z. Nain, Z. Ahmed, A. Raza, M. Ali, U. Ahmad, F. U. Arif, M. Nasir, M. Qasim, Muhammad Rizwan Shafiq, & S. Iqbal, Trans.). (2026). Journal of Agriculture and Veterinary Science, 5(1), 73-83. https://doi.org/10.55627/agrivet.005.01.01854