EMBRYOGENIC CALLUS INDUCTION AND REGENERATION EFFICIENCY IN DATE PALM
DOI:
https://doi.org/10.55627/pbulletin.003.02.0977Abstract
The date palm (Phoenix dactylifera) is widely cultured for its medicinal and nutritional value as an edible fruit. Traditional vegetative propagation of date palm is time-consuming, requiring several years. However, modern biotechnological techniques, including plant tissue culture, have revolutionized mass production by significantly reducing the time required. Plant tissue culture involves using small tissues to grow and regenerate entire plants in an artificial (In Vitro) environment under sterile laboratory conditions. This study aimed to enhance the regeneration efficiency and induction of embryogenic callus in date palm. Callus induction was carried out using suckers of the date palm on Murashige and Skoog (MS) media with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with different plant growth regulators (PGRs), including 2-isopentenyladenine (2iP), naphthalene acetic acid (NAA), benzylaminopurine (BAP), and indole acetic acid (IAA). Experiments were conducted in triplicate, with each hormonal treatment including three explants per replication. The best media combination for callus induction was MS + 2,4-D, 1.5 mgL-1 + 2ip 4 mgL-1 for variety Dakki and MS + 2,4-D 1.5mgL-1 + 2ip 5 mgL-1 for variety Hillavi in datepalm. Callus regeneration for Dakki was observed under treatment 3 containing MS + 3 mgL-1IAA + 1.5mgL-1 BAP took minimum days for callus regeneration. Hillavi took minimum days for callus regeneration under treatment 9 contains MS + 2ip 6 mgL-1 + IAA 1mgL-1. Hillavi took minimum days for shoot formation under treatment 2 contains MS + 1.5mg/L BAP + 1.5 mg/L IAA and Dakki took minimum days under treatment 8 containing different harmonal combination MS + 1mg/L IAA + 5 mg/L 2ip. Dakki took a minimum of days for new root formation under treatment 9 MS + 5 mgL -1 NAA + 4 mgL-1 IBA+4 mgL-1 IAA and Hillavi took minimum days under treatment 2 MS + 1 mgL -1 IAA + 1mgL-1 IBA. Data were analyzed using analysis of variance (ANOVA) under a completely randomized design, and means were compared using the least significant difference (LSD) test.
References
REFERENCES
1. Al-Karmadi, A., and A. I. Okoh. 2024. An Overview of Date (Phoenix dactylifera) Fruits as an Important Global Food Resource. Foods. 13(7): 1024.
2. Ahmad, M. and A.A. Mirani. 2008. Development and performance of a Solar-Cum-Gas fired dates dryer. AMA. 39(4): 59-63.
3. Shahsavar, A. R., and A. Shahhosseini. 2022. The metaxenia effects of different pollen grains on secondary metabolites enzymes and sugars of ‘Piarom’date palm fruit. Sci. Rep. 12(1):10058.
4. Al-Khalifah, N. and E. Askari. 2011. Growth abnormalities associated with micropropagation of date palm. Afr. J. Biotechnol. 205-219.
5. Al-Khayri, J.M. and P.M. Naik. 2017. Date palm micropropagation: Advances and applications. Cienc. Agrotecnologia. 41: 347-358.
6. Al-Najm, A., S. Brauer, R. Trethowan and N. Ahmad. 2018. Optimisation of in'vitro micropropagation'of several date palm cultivars. Aust. J. Crop Sci. 12: 1937-1949.
7. Cohen, Y., R. Korchinsky and E. Tripler. 2004. Flower abnormalities cause abnormal fruit setting in tissue culture-propagated date palm (Phoenix dactylifera L.). J. Hortic. Sci. Biotechnol. 79:1007-1013.
8. Dharmasiri, N. and M. Estelle. 2004. Auxin signaling and regulated protein degradation. Trends Plant Sci. 9: 302-308.
9. Dransfield, J., N.W. Uhl, C.B. Asmussen, W.J. Baker, M.M. Harley and C.E. Lewis. 2005. A new phylogenetic classification of the palm family, Arecaceae. Kew Bull. 559-569.
10. Weyers, J. D., and N. W. Paterson. 2001. Plant hormones and the control of physiological processes. New Phytol. 152(3): 375-407.
11. Johnson, D. 2011. Introduction: date palm biotechnology from theory to practice. Date palm biotechnology.1-11.
12. Lu, C.-Y., S.F. Chandler and I.K. Vasil. 1984. Somatic embryogenesis and plant regeneration from cultured immature embryos of rye (Secale cereale L.). J. Plant Physiol. 115: 237-244.
13. Masmoudi-Allouche, F., B. Meziou, W. Kriaâ, R. Gargouri-Bouzid and N. Drira. 2010. In vitro flowering induction in date palm (Phoenix dactylifera L.). J. Plant Growth Regul. 29.35-43.
14. Mazri, M.A. 2015. Role of cytokinins and physical state of the culture medium to improve in vitro shoot multiplication, rooting and acclimatization of date palm (Phoenix dactylifera L.) cv. Boufeggous. J. Plant Biochem. Biotechnol. 24: 268-275.
15. Metwally, H., A. El-Bana, Y. Tahany and Y. Diab. 2019. Evaluation of Some Selected Seeded Date Palms and Determination of its Fruit Characteristics under Dakhla Oasis Conditions-New Valley-Egypt. Middle East J. Appl. Sci. 9: 711-726.
16. Riad, M. 1996. The date palm sector in Egypt. CIHEAM-Options Mediterr. 28: 45-53.
17. Teixeira, J., M. Söndahl, T. Nakamura and E. Kirby. 1995. Establishment of oil palm cell suspensions and plant regeneration. Plant Cell Tissue Organ Cult. 40: 105-111.
18. Tisserat, B. 1982. Factors involved in the production of plantlets from date palm callus cultures. Euphytica. 31: 201-214.
19. Tisserat, B. and D. Demason. 1980. A histological study of development of adventive embryos in organ cultures of Phoenix dactylifera L. Ann. Bot. 46: 465-472.
20. Wrigley, G. 1995. Date palm (Phoenix dactylifera L.) In: Smartt J, Simmonds NW (eds.) Genet. Resour. Crop Evol. 399-403.
21. Zaid, A., B. El-Korchi and H. Visser. 2011. Commercial date palm tissue culture procedures and facility establishment. Afr. J. Biotechnol. 2011. 137-180.
22. Shahrour, W. G., M. A. Shatnawi, R. A. Shibli, M. Al-Alawi, S. M. Abubaker, M. Majdalawi, A. K. Alkawaldeh. 2024. Callus induction, shoot regeneration, and conservation of in vitro grown date palm (Phoenix dactylifera). "Braz. J. Biol.84: e284231.
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