Investigation on Water Borne Protozoan Parasites in Drinking Water of Quetta and Sohbat Pur Districts of Balochistan

Tehmina  Sajid; Abdul  Razzaq; Asim Shamim; Sadaf  Shahid; Dad Muhammad  Anwar; Zahida  Fatima; Munib  Hussain; Muhammad Essa  Kakar; Muhammad  Bilal; Shakeel Ahmed  Bugti; Muhammad  Ramzan; Sajid Ali  Khosa

doi:10.55627/agrivet.03.01.0371

Authors

Tehmina Sajid Department of Zoology Sardar Bahadur Khan Women’s University Baluchistan Quetta
Abdul Razzaq Animal Science Division, Pakistan Agriculture Research Council Islamabad
Asim Shamim Department of Pathobiology, Faculty of Veterinary and Animal Sciences University of Poonch Rawalakot, Azad Kashmir
Sadaf Shahid Department of Zoology Sardar Bahadur Khan Women’s University Baluchistan Quetta
Dad Muhammad Anwar Veterinary Research Institute, Quetta, Balochistan
Zahida Fatima National Reference Laboratory for Poultry Disease, NARC Islamabad
Munib Hussain Animal Health Program, ASI, NARC Islamabad
Muhammad Essa Kakar Disease Investigation Laboratory, Livestock and Dairy Development Department, Balochistan
Muhammad Bilal Disease Investigation Laboratory, Livestock and Dairy Development Department, Balochistan
Shakeel Ahmed Bugti Disease Investigation Laboratory, Livestock and Dairy Development Department, Balochistan
Muhammad Ramzan Disease Investigation Laboratory, Livestock and Dairy Development Department, Balochistan
Sajid Ali Khosa Veterinary Research Institute, Quetta, Balochistan

DOI:

https://doi.org/10.55627/agrivet.03.01.0371

Keywords:

Baluchistan, Drinking water, Protozoa, Stool samples

Abstract

Current study was aimed to investigate the prevalence of water-borne protozoa in drinking water and stool samples from suspected intestinal effected patients in districts Quetta and Sohbat Pur, Balochistan. For this purpose, 200 water samples were collected from different water sources of the selected study areas. Collected samples were transported to the Sardar Bahadur Khan Woman University for further processing. Similarly, 200 human stool samples were also collected and transported to the Bolan Medical complex microbiology laboratory for further processing. Staining of stool samples was done by iodine and modified acid fast stain and examined under microscope. Culturing of positive stool samples weas carried out using lock egg media to culture Entamoeba histolytica. Overall, 15% protozoan prevalence was recorded from the water samples collected among the selected study areas where higher prevalence was observed in Sohbat Pur (20%) followed by Quetta (10%). Among water bodies higher prevalence was observed in canal system (40%). Protozoan distribution was recorded higher in May and August (17.5%) in water samples. Overall, 22% protozoan prevalence was recorded from the stool samples and higher prevalence was recorded in Sohbat Pur (27%) followed by Quetta (18%). Children from age group 11-20 years were found more susceptible as compared to adults. Based on month wise prevalence, higher prevalence was recorded in August (27.5%) and lowest in July (17.5%). Entamoeba histolytica was found more prevalent (17%) as compared to Giardia lamblia (5.5%). Statistical analysis shows there is no significant difference (P>0.05) among water and stool samples of Quetta and Sohabat Pur district of Balochistan. Further importance of the current study is discussed.

References

Aina, T., J. Davila, J. Hardoy and D. Satterthwaite. 2005. Environment and Urbanization–Environmental Problems in Third World CitiesRussell Press. Nottingham, United Kingdom. pp. 4-84.

Al-Idrrise, S., S. A. A. A. Al-Azizz, G. Y. Al-Emara and A. S. Al-Badran. 2008. Culture of Entamoeba histolytica in vitro. Al-Qadisiyah Journal of Veterinary Medicine Sciences, 7: 1-6.

Altizer, S., A. Dobson, P. Hosseini, P. Hudson, M. Pascual and P. Rohani. 2006. Seasonality and the dynamics of infectious diseases. Ecology letters, 9: 467-84.

Amin, O. M. 2002. Seasonal prevalence of intestinal parasites in the United States during 2000. The American journal of tropical medicine and hygiene, 66: 799-803.

Amin, O. M. 2006. The epidemiology of Blastocystis hominis in the United States. Research Journal Parasitology, 1: 1-10.

Ayaz, S., S. Khan, S. N. Khan, F. Bibi, S. Shamas and M. Akhtar. 2011. Prevalence of zoonotic parasites in drinking water of three districts of Khyber Pakhtunkhwa Province, Pakistan. Pakistan journal of life and social sciences, 9: 67-69.

Bakir, B., M. Tanyuksel, F. Saylam, S. Tanriverdi, R. Engin Araz, A.-K. Hacim and M. Hasde. 2003. Investigation of waterborne parasites in drinking water sources of Ankara, Turkey. Journal of Microbiology, 41: 148-51.

Bernawi, A. A., S.-E. M. Omar and S. E. Kti. 2013. Prevalence of Giardia lamblia in humans visited central laboratory of Sebha province. International Journal of Engineering Sciences and Innovated Technology, 2: 2319-5967.

Bethony, J., S. Brooker, M. Albonico, S. M. Geiger, A. Loukas, D. Diemert and P. J. Hotez. 2006. Soil-transmitted helminth infections: Ascariasis, trichuriasis, and hookworm. The lancet, 367: 1521-32.

Chaudhry, Z. H., M. Afzal and M. A. Malik. 2004. Epidemiological factors affecting prevalence of intestinal parasites in children of Muzaffarabad district. Pakistan journal of Zoology, 36: 267-71.

Costa, J. d. O., J. A. Resende, F. F. Gil, J. F. G. Santos and M. A. Gomes. 2018. Prevalence of Entamoeba histolytica and other enteral parasitic diseases in the metropolitan region of Belo Horizonte, Brazil. A cross-sectional study. São Paulo Medical Journal, 136: 319-23.

Dash, N., M. Al-Zarouni, K. Anwar and D. Panigrahi. 2010. Prevalence of intestinal parasitic infections in Sharjah, United Arab Emirates. Human Parasitic Diseases, 2: 21.

Feenstra, S., R. Hussain and W. v. d. Hoek. 2000. Prevalence of intestinal parasites in the southern Punjab, Pakistan. IWMI Pakistan Report.

Ghasemi, A. 2012. Effect of irrigation withdrawal and N fertilization rates on yield and agronomical traits of triticale in Birjand, Iran, Islamic Azad University.

Hussain, S., M. Raza and S. Naeem. 1997. Prevalence of intestinal parasites in Northern areas of Pakistan (Baltistan Division-Skardu). Biomedica, 13: 60-64.

Kaplan, R. 1992. Microscopic examination of faecal specimens: Permanent stained smears (Trichrome) Clinical microbiology procedures handbook.

Khalil, A., M. Jan, R. Imran, N. Shuja and G. Shah. 2012. Prevalence of intestinal parasitic pathogens among gastroenteritis patients in District Gilgit, Gilgit-Baltistan, Pakistan. Pakistan journal of Zoology, 44: 1059-63.

Khan, F. J. and Y. Javed. 2007. Delivering access to safe drinking water and adequate sanitation in Pakistan. Pakistan Instiute of Development Economics.

Khan, S., M. Shahnaz, N. Jehan, S. Rehman, M. T. Shah and I. Din. 2013. Drinking water quality and human health risk in Charsadda district, Pakistan. Journal of cleaner production, 60: 93-101.

Kwakye-Nuako, G., P. Borketey, I. Mensah-Attipoe, R. Asmah and P. Ayeh-Kumi. 2007. Sachet drinking water in Accra: the potential threats of transmission of enteric pathogenic protozoan organisms. Ghana medical journal, 41: 62-67.

Masangkay, F. R., G. D. Milanez, A. Tsiami, F. Z. Hapan, V. Somsak, M. Kotepui, J. Tangpong and P. Karanis. 2020. Waterborne protozoan pathogens in environmental aquatic biofilms: Implications for water quality assessment strategies. Environmental Pollution, 259: 113903.

Morgan, f. U., L. Pallant, B. Dwyer, D. Forbes, G. Rich and R. Thompson. 1998. Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: Clinical trial. Journal of Clinical Microbiology, 36: 995-98.

Nematian, J., E. Nematian, A. Gholamrezanezhad and A. A. Asgari. 2004. Prevalence of intestinal parasitic infections and their relation with socio-economic factors and hygienic habits in Tehran primary school students. Acta tropica, 92: 179-86.

Oguntibeju, O. 2006. Prevalence of intestinal parasites in HIV-positive/AIDS patients. The Malaysian Journal of Medical Sciences, 13: 68-73.

Pham Duc, P., H. Nguyen-Viet, J. Hattendorf, J. Zinsstag, P. Dac Cam and P. Odermatt. 2011. Risk factors for Entamoeba histolytica infection in an agricultural community in Hanam province, Vietnam. Parasites and Vectors, 4: 1-9.

Ramírez-Castillo, F. Y., A. Loera-Muro, M. Jacques, P. Garneau, F. J. Avelar-González, J. Harel and A. L. Guerrero-Barrera. 2015. Waterborne pathogens: Detection methods and challenges. Pathogens, 4: 307-34.

Sente, C., J. Erume, I. Naigaga, J. Mulindwa, S. Ochwo, P. K. Magambo, B. G. Namara, C. D. Kato, G. Sebyatika and K. Muwonge. 2016. Prevalence of pathogenic free-living amoeba and other protozoa in natural and communal piped tap water from Queen Elizabeth protected area, Uganda. Infectious diseases of poverty, 5: 84-97.

Shaikh, G., R. Begum, A. Hussain and R. Shaikh. 2009. Prevalence of intestinal protozoan and helminth parasites in Sukkur, Sindh. Sindh University Research Journal, 41: 53-58.

Siddiqui, M. S., M. Amin, M. Amber, M. Abbas, S. K. Sherwani, M. W. Malik and M. Husaain. 2012. Association of socio-economic features, hygienic status, age groups and gender with prevalence of waterborne diseases in Rawalpindi and Islamabad. Science, Technology and Development, 31: 219-26.

Siwila, J., F. Mwaba, N. Chidumayo and C. Mubanga. 2020. Food and waterborne protozoan parasites: The African perspective. Food and Waterborne Parasitology, 20: e00088.

Soomro, M., M. Khokhar, W. Hussain and M. Hussain. 2011. Drinking water Quality challenges in Pakistan Pakistan Council of Research in Water Resources. Lahore, Pakistan. pp. 17-28.

Tasawar, Z., S. Kausar and M. Lashari. 2010. Prevalence of Entamoeba histolytica in humans. Pakistan journal of pharmaceutical sciences, 23.

Uddin, M. S. and K. Kurosawa. 2011. Effect of chemical nitrogen fertilizer application on the release of arsenic from sediment to groundwater in Bangladesh. Procedia Environmental Sciences, 4: 294-302.

WHO. 2017. Regional Office for the Eastern mediterranean Health education: theoretical concepts, effective strategies and core competencies. World Health Organization.

Young, M., C. Wolfheim, D. R. Marsh and D. Hammamy. 2012. World health organization/United Nations children's fund joint statement on integrated community case management: an equity-focused strategy to improve access to essential treatment services for children. The American journal of tropical medicine and hygiene, 87: 6-19.

Yousefi, Z. and R. Mohammadpour. 2007. Contamination of drinking water supplies in Mazandaran-Iran. Journal of Kermanshah University of Medical Sciences, 10: 378-88.

Yusuf, N. A., Y. M. Yusri, N. Ismail and I. Vythilingam. 2007. Prevalence of intestinal protozoa in an aborigine community in Pahang, Malaysia. Tropical Biomedicine, 24: 55-62.

Zeb, A., A. W. Qureshi, L. Khan, A. Mansoor, S. Ullah and M. Irfan. 2018. Prevalence of Entamoeba histolytica in district Buner Khyber Pakhtunkhwa, Pakistan. Journal of Entomology and Zoology Studies, 6: 185-88.