The Influence of Glucose and Fructose as Energy Source in Semen Extender on the Quality of Chilled Canine Semen Stored at 4 °C
DOI:
https://doi.org/10.55627/agrivet.004.02.01015Keywords:
Motility, Acrosomal Integrity, Fructose-Based Semen Extender, Glycerol-Based Semen ExtenderAbstract
The experiment was designed to assess the effects of alternative energy sources on chilled semen, extended for use over a longer period of time. Fructose and glucose were used as energy sources to evaluate the sperm viability when semen is chilled for a longer period of time. Five dogs were selected, and semen was collected at five-day intervals. Extender was added only to the sperm-rich fraction and evaluated for seminal parameters and sperm morphology at 0, 24, 48, and 96 hours at 4°C. The results showed that the addition of glucose to the semen extender resulted in sperm motility of 70.6% ± 2.54% and acrosomal integrity of 47.6% ± 3.11%. In comparison, fructose resulted in 74.2% ± 2.50% motility and 52% ± 3.10% acrosomal integrity after 24 hours of cold storage. These findings indicate significantly improved sperm motility and acrosomal integrity with the fructose-based semen extender compared to glucose. In conclusion, the addition of fructose to the semen extender has a positive influence on sperm quality.
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