Effect of Different Concentrations of Aloe vera Gel on Seed Germination and Growth of Moringa (Moringa oleifera)

Authors

  • Muzafaruddin Chachar Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam
  • Saba Ambreen Memon Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam
  • Tanveer Fatima Miano Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam
  • Sadaruddin Chachar Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, Tandojam. , College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, China.
  • Memoona Islam Majeedano Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam.
  • Zaheer Ahmed Chachar Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam.
  • Saeed Ahmed Chachar Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam.
  • Raheem Ullah Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam.
  • Sana Shazia Jiskani Department of Horticulture, Faculty of Crop Production, Sindh Agriculture University, Tandojam. , National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, China.
  • Tahir Mehmood Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

DOI:

https://doi.org/10.55627/agrivet.004.03.1502

Keywords:

Moringa, Aloe vera, Priming, Natural Biostimulant, Seed Germination and Seedling Vigor

Abstract

Moringa (Moringa oleifera) cultivation is limited germination and early seedling growth challenges. Aloe vera gel, a natural biostimulant, shows potential, but its effects on Moringa are underexplored. The primary objective of this investigation was to identify sustainable ways to increase the germination as well as seedling vigor of Moringa. The study had taken place at the Horticulture Department of Sindh Agriculture University in Tandojam during the fall of 2024. The findings from this research investigates the impact of varying concentrations of Aloe vera gel on the germination and growth of Moringa (Moringa oleifera). The experiment was laid out in a CRD with six treatments, replicated three times. T0 (control), T1 priming (distilled water), T2 (1% Aloe vera gel), T3 (30% Aloe vera gel), T4 (60% Aloe vera gel), and T5 (100% Aloe vera gel). Key germination and growth parameters, including germination percentage, time to germination, germination index, seedling vigor index, number of branches per plant, shoot and root fresh biomass, seedling quality index, plant height, and sturdiness quotient were evaluated in this study. Findings showed that 100% Aloe vera gel (T5) significantly improved germination percentage (82.66%), germination index (4.99), vigor index (2230), plant height (42.11 cm), and number of branches (14.66), while control treatment consistently produced the lowest values. Interestingly, shoot and root biomass, as well as seedling quality index, were maximized at lower concentrations (1% Aloe vera gel). These results highlight the potential of Aloe vera gel as a sustainable and natural biostimulant to overcome germination challenges in Moringa. Future studies should focus on field-scale validation, optimization of gel concentrations, and integration with other eco-friendly priming techniques to establish standardized protocols for large-scale Moringa cultivation.

Author Biography

  • Sadaruddin Chachar, Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, Tandojam., College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, China.

     

     

References

Aamur, G. R., T. F. Miano, M. Chachar, M. I. Majeedano, M. R. Tunio, S. S. Jiskani, R. Ullah and Z. A. Chachar. 2025. Effect of macronutrient levels on growth and development of spinach (Spinacia oleracea L.). Integrative Plant Biotechnology, 3 (3), 231–239.

Abbas, S., M. Zaglool, E. El-Ghadban, A. El-Kareem and A. Waly. 2016. Effect of foliar application with aloe leaf extract (ALE) on vegetative growth, oil percentage and anatomical leaf structure of sage (Salvia officinalis L.) plant under sand soil conditions. HortScience Journal of Suez Canal University, 5(1), 9–14.

Abdul Baki, A. A. and J. D. Anderson. 1973. Vigor determination in soybean seed by multiple criteria. Crop Science, 13(6), 630–633.

Ahmad, S. K., K. A. Hammam and A. A. Amer. 2014. Effect of bio-fertilization and some plant extracts on the growth, yield, and chemical constituents of basil plant. Journal of Plant Production, 5(2), 193–210.

Alegbeleye, O. O. 2018. How functional is Moringa oleifera? A review of its nutritive, medicinal, and socioeconomic potential. Food and Nutrition Bulletin, 39(1), 149–170.

AOSA. 1983. Seed vigor testing handbook (Contribution No. 32 to Handbook on Seed Testing). Association of Official Seed Analysts.

Çavuşoğlu, D., S. Tabur and K. Çavuşoğlu. 2016. The effects of Aloe vera L. leaf extract on some physiological and cytogenetical parameters in Allium cepa L. seeds germinated under salt stress. Cytologia, 81(1), 103–110.

Chachar, S. A., N. A. Wahocho, M. Chachar, M. I. Majeedano, Z. A. Chachar, S. S. Jiskani, M. A. Wagan and R. Ullah. 2025. Effects of Basil Leaf Extract Priming on Seed Germination and Seedling Growth of Okra (Abelmoschus esculentus). Integrative Plant Biotechnology, 3(3), 193-204.

Chatterjee, P., C. Bodhisattwa and N. Subhangkar. 2013. Significant pharmacological activities of Aloe vera plant. Mintage Journal of Pharmaceutical and Medical Sciences, 3(1), 21–24.

Dagne, E., D. Bisrat, A. Viljoen and B.-E. Van Wyk. 2000. Chemistry of Aloe species. Current Organic Chemistry, 4(10), 1055–1078.

Dickson, A., A. L. Leaf and J. F. Hosner. 1960. Quality appraisal of white spruce and white pine seedling stock in nurseries. The Forestry Chronicle, 36(1), 10–13.

DongZhi, L., E. Tsuzuki, Y. Sugimoto, D. YanJun, M. Matsuo and H. Terao. 2004. Allelopathic effects of aqueous Aloe vera leaf extracts on selected crops. Allelopathy Journal, 13(1), 67–74.

Dzib-Ek, G., E. Villanueva-Couoh, R. Garruña-Hernández, S. Vergara Yoisura and A. Larqué-Saavedra. 2021. Effect of salicylic acid on germination and root growth of tomato. Mexican Journal of Agricultural Sciences, 12(4), 735–740.

El Bilali, H., I. Dan Guimbo, R. K. Nanema, H. Falalou, Z. Kiebre, V. M. Rokka, S. R. F. Tietiambou, J. Nanema, L. Dambo, F. Grazioli, A. K. Naino Jika, M. Gonnella and F. Acasto. 2024. Research on Moringa (Moringa oleifera Lam.) in Africa. Plants, 13(12), 1613.

El Sherif, F. 2017. Aloe vera leaf extract as a potential growth enhancer for Populus trees grown under in vitro conditions. American Journal of Plant Biology, 2(4), 101–105.

El-Shayeb, N. S. 2009. Physiological studies on Oenothera biennis (bio-fertilizer and plant extracts). Faculty of Agriculture, Moshtohor, Benha University.

Falowo, A. B., F. E. Mukumbo, E. M. Idamokoro, J. M. Lorenzo, A. J. Afolayan and V. Muchenje. 2018. Multi-functional application of Moringa oleifera Lam. in nutrition and animal food products: A review. Food Research International, 106(1), 317–334.

Gopalakrishnan, L., K. Doriya and D. S. Kumar. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5(2), 49–56.

Hamouda, A. M. A., D. M. Hendi and O. F. Abu-El-Leel. 2012. Improving basil growth, yield and oil production by Aloe vera extract and active dry yeast. Egyptian Journal of Horticulture, 39(1), 45–71.

Hanafy, M. S., F. M. Saadawy, S. M. N. Milad and R. M. Ali. 2012. Effect of some natural extracts on growth and chemical constituents of Schefflera arboricola plants. Journal of Horticultural Science and Ornamental Plants, 4(1), 26–33.

Hao, P. M., L. P. T. Quoc, P. N. M. Thu, N. T. T. Dao and L. B. Vy. 2024. A comprehensive review of Moringa oleifera Lam.: A valuable plant in food and medicine. International Journal of Agricultural Technology, 20(5), 1899–1916.

Hemalatha, G., K. Manoj Kumar and U. Gowthamy. 2024. Effect of seed priming studies on seed germination in groundnut (Arachis hypogea L.). International Journal of Advanced Biochemistry Research, 8(6), 165–170.

Herman-Lara, E., J. Rodríguez-Miranda, S. Ávila-Manrique, C. Dorado-López, M. Villalva, L. Jaime, S. Santoyo and C. E. Martínez-Sánchez. 2024. In vitro antioxidant, anti-inflammatory activity and bioaccessibility of ethanolic extracts from Mexican Moringa oleifera leaf. Foods, 13(17), 2709.

Hernández-Cruz, L. R., R. Rodríguez-García, L. D. Rodríguez, J. Janik and A. Whipkey. 2002. Aloe vera response to plastic mulch and nitrogen. Environmental Science and Pollution Research, 9(special issue), 570–574.

Imran, S., I. Afzal, M. Amjad, A. Akram, K. M. Khawar and S. Pretorius. 2014. Seed priming with aqueous plant extracts improved seed germination and seedling growth under chilling stress in lentil (Lens culinaris Medik). Acta Advances in Agricultural Sciences, 2(1), 58–69.

Janmohammadi, M., P. M. Dezfuli and F. Sharifzadeh. 2008. Seed invigoration techniques to improve germination and early growth of inbred line of maize under salinity and drought stress. Journal of Applied Plant Physiology, 34(3–4), 215–226.

Jayanthi, M., S. K. Garg, P. Yadav, A. K. Bhatia and A. Goel. 2015. Some newer marker phytoconstituents in methanolic extract of Moringa oleifera leaves and evaluation of its immunomodulatory and splenocytes proliferation potential in rats. Indian Journal of Pharmacology, 47(5), 518–523.

Kaur, A., A. Singh and R. Monga. 2020. Seed germination enhancement through breaking seed dormancy: A review in tropical and temperate tree species. International Journal of Current Microbiology and Applied Sciences, 9(9), 1673–1688.

Luna, R. K. and N. Chamouli. 2006. Effect of root trainer size on the quality of seedling production in Albizia proscera (Roxb.) Benth., Eucalyptus teriticornis Sm., and Acacia catechu Wild. Annals of Forestry, 14(2), 184–193.

Mady, A. (2008). Effect of certain medicinal plant extracts on growth, yield and metabolism of some medicinal and aromatic plants (M.Sc. thesis). Faculty of Science, Al-Azhar University.

Mahato, D. K., R. Kargwal, M. Kamle, B. Sharma, S. Pandhi, S. Mishra and P. Kumar. 2022. Ethnopharmacological properties and nutraceutical potential of Moringa oleifera. Phytomedicine Plus, 2(1), 100162.

Meireles, D., J. Gomes, L. Lopes, M. Hinzmann and J. Machado. 2020. A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: Integrative approach on conventional and traditional Asian medicine. Advances in Traditional Medicine, 20(4), 495–515.

Moremane, M. M., B. Abrahams and C. Tiloke. 2023. Moringa oleifera: A review on the antiproliferative potential in breast cancer cells. Current Issues in Molecular Biology, 45(8), 6880–6902.

Ni, Y. and I. R. Tizard. 2004. Analytical methodology: The gel-analysis of aloe pulp and its derivatives. In T. Reynolds (Ed.), Aloes: The genus Aloe (pp. 129–144). CRC Press.

Ni, Y., D. Turner, K. Á. Yates and I. Tizard. 2004. Isolation and characterization of structural components of Aloe vera L. leaf pulp. International Immunopharmacology, 4(14), 1745–1755.

Nouman, W., S. M. A. Basra, A. Yasmeen, T. Gull, S. B. Hussain, M. Zubair and R. Gul. 2014. Seed priming improves the emergence potential, growth and antioxidant system of Moringa oleifera under saline conditions. Plant Growth Regulation, 73(3), 267–278.

Nour El-Din, T. 2005. Physiological studies on marjoram plants (Majorana hortensis) (M.Sc. thesis). Department of Ornamental Horticulture, Faculty of Agriculture, Zagazig University (Banha Branch).

Nuapia, Y., E. Cukrowska, H. Tutu and L. Chimuka. 2020. Statistical comparison of two modeling methods on pressurized hot water extraction of vitamin C and phenolic compounds from Moringa oleifera leaves. South African Journal of Botany, 129(1), 9–16.

Padmaja, C. K., B. Kowsalya and C. Seethalakshmi. 2007. Efficacy of Aloe vera (L.) leaf powder as a biostimulant in enhancing the growth and yield of lady’s finger (Abelmoschus esculentus L.). Research on Crops, 8(2), 395–397.

Pandey, A., R. D. Pandey, P. Tripathi, P. P. Gupta, J. Haider, S. Bhatt and A. V. Singh. 2012. Moringa oleifera Lam. (Sahijan) – A plant with a plethora of diverse therapeutic benefits: An updated retrospection. Medicinal and Aromatic Plants, 1(1), 1–8.

Ramachandra, C. T. and P. S. Rao. 2008. Processing of Aloe vera leaf gel: A review. American Journal of Agricultural and Biological Sciences, 3(2), 502–510.

Raman, R. P. V., R. S. Rita, B. C. Mondal and S. K. Singh. 2013. Effect of Aloe vera and clove powder supplementation on carcass characteristics, composition and serum enzymes of Japanese quails. Veterinary World, 8(5), 664–668.

René, N. P., D. C. Tonessia, D. F. Soko, E. F. Soumahin, K. D. N’goran and D. S. Akaffou. 2024. Influence of Aloe vera gel on germination and early growth of tomato (Solanum lycopersicum) seedlings in the nursery. Journal of Experimental Agriculture International, 46(10), 91–102.

Reynolds, T. and A. C. Dweck. 1999. Aloe vera leaf gel: A review update. Journal of Ethnopharmacology, 68(1–3), 3–37.

Rocchetti, G., F. Blasi, D. Montesano, S. Ghisoni, M. C. Marcotullio, S. Sabatini, C. Lina and L. Lucini. 2019. Impact of conventional and non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves. Food Research International, 115(1), 319–327.

Rodríguez-Larramendi, L. A., M. G. Ramírez, M. A. Gómez-Rincón, F. Guevara-Hernández, M. Á. Salas-Marina and A. Gordillo-Curiel. 2017. Effects of salicylic acid on germination and early growth of bean seedlings (Phaseolus vulgaris L.). Revista de la Facultad de Agronomía (LUZ), 34(3), 253–269.

Roller, K. J. 1977. Suggested minimum standards for containerized seedlings in Nova Scotia. Canadian Forestry Service, Department of Environment, Information Report M-X-69.

Sahu, K. P., D. D. Giri, R. Singh, P. Pandey, S. Gupta, A. K. Shrivastava and D. K. Pandey. 2013. Effect of Aloe vera on some annual plants. Scientific Research of Pharmacology and Pharmacy, 4(6), 599–610.

Schaffer, M., D. A. Grant, K. Berge and N. Y. D. Ankrah. 2024. Moringa reduces glucose levels and alters Wolbachia abundance in Drosophila melanogaster. Microbiology Research, 15(3), 1870–1879.

Sreeja, M., P. Jayasri, N. Keerthi, J. Yeshashwini and J. Praveen. 2021. Moringa oleifera: A review on nutritive importance and its potential use as nutraceutical plant. Journal of Medicinal Plants, 9(2), 15–17.

Statistix. (2006). Statistix 8 user guide, version 1.0. Analytical Software.

Suleiman, M. K., N. R. Bhat, M. S. Abdal, S. Zaman, R. R. Thomas and S. Jacob. 2008. Germination studies in Nitraria retusa (Forssk.) Asch. Middle-East Journal of Scientific Research, 3(4), 211–213.

Sumantra, I. K. and I. K. Widnyana. 2011. Effectiveness of Aloe vera gel and coconut water as a bioregulator on seed germination of Dendrobium orchid. Jurnal Agrimeta, 1(1), 1–9.

Surjushe, A., R. Vasani and D. Saple. 2008. Aloe vera: A short review. Indian Journal of Dermatology, 53(4), 163–166.

Tamuk, G. and B. Singh. 2022. Enhancing seed germination in Altingia excelsa Noronha pre-treated by natural plant extracts under laboratory conditions at Pasighat in Arunachal Pradesh, India. The Pharma Innovation Journal, 11(12), 357–362.

Thompson, B. E. 1985. Seedling morphological evaluation: What you can tell by looking. In M. L. Duryea (Ed.), Proceedings: Evaluating seedling quality—principles, procedures, and predictive abilities of major tests (pp. 59–71). Forest Research Laboratory, Oregon State University.

Zeljković, S., N. Parađiković, J. D. Gidas, E. Mladenović and A. Vujošević. 2020. The effect of water extract of Aloe vera (L.) Burm. f. on germination and growth of scarlet sage. In XI International Scientific Agricultural Symposium “Agrosym 2020” Proceedings (pp. 262–267). University of East Sarajevo.

Downloads

Published

2025-10-17

Issue

Section

Research Articles

How to Cite

Effect of Different Concentrations of Aloe vera Gel on Seed Germination and Growth of Moringa (Moringa oleifera) (M. Chachar, S. A. Memon, T. F. Miano, S. Chachar, M. I. Majeedano, Z. A. Chachar, S. A. Chachar, R. Ullah, S. S. Jiskani, & T. Mehmood, Trans.). (2025). Journal of Agriculture and Veterinary Science, 4(3), 285-297. https://doi.org/10.55627/agrivet.004.03.1502

Similar Articles

11-20 of 32

You may also start an advanced similarity search for this article.