Validation and Development of Microkjeldahl Distillation Method for Amino Acid Determination
DOI:
https://doi.org/10.55627/agrivet.004.03.01638Keywords:
Amino acid, Microkjeldahl, Distillation, Development, ValidationAbstract
To determine the amounts of nutrients they contain, fertilizer samples are tested; however, the results vary based on the techniques. Therefore, developing and validating a technique for Amino acid determination using Microkjeldahl Distillation Method was the main goal of this study. The Soil & Water Testing Laboratory (SWTL) Dera Ghazi Khan (accredited ISO/IEC, 17025:2017) approved the Microkjeldahl Distillation Method for measuring the amounts of Amino acid. The validation method included bias, recovery, limit of quantification, limit of detection, repeatability, and reproducibility and T-test was used for reproducibility. The limits for detection and quantification were, respectively, 0.74% and 2.46%. The reproducibility indicated T-calculated values of 0.862, which were lower than the T-tabulated (2.262), with repeatability RSD of 3.151%. The Amino acid recovery rate was 99.40%. Vietnam's findings (QUATEST3 www.quatest3.com.vn) had Z-scores that were within the acceptable range. The estimated values of Amino acid and their genuine values are strongly correlated, as indicated by recovery (i.e 0.99.40%). This outcome shows that the approach performed at its peak. Because all of the parameters worked well and produced accurate findings according to accepted standards. Therefore, the method might be used to determine the amount of Amino acid in fertilizers really well.
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Copyright (c) 2025 Muhammad Bilal, Rehmat Ullah, Muhammad Akram Qazi, Fareeha Habib, Farhat Bashir, Sobia Noor, Abid Niaz, Amir Afzal
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