The Effects of Human Recombinant Erythropoietin on Various Hemato-biochemical Parameters in Nephrectomized Rabbits

Authors

  • Muhammad Asif Department of Veterinary Surgery, University of Veterinary and Animal Sciences, Lahore
  • Hamid Akbar Department of Veterinary Surgery, University of Veterinary and Animal Sciences, Lahore
  • Waqas Haroon Department of Anatomy & Histology, University of Veterinary and Animal Sciences, Lahore
  • Sana Saeed Department of Anatomy & Histology, University of Veterinary and Animal Sciences, Lahore
  • Samia Mushtaq Department of Veterinary Sciences, Azad Jammu and Kashmir, University of Bhimber
  • Muhammad Ali Department of Pathobiology, University of Layyah, Layyah
  • Asma Fatima Department of Veterinary Surgery, University of Veterinary and Animal Sciences, Lahore
  • Muhammad Zahir Department of Veterinary Surgery, University of Veterinary and Animal Sciences, Lahore
  • Abdul Aziz Department of Veterinary Clinical Sciences, University of Poonch, Rawalakot
  • Fazal Rehman Department of Veterinary Surgery, University of Veterinary and Animal Sciences, Lahore

DOI:

https://doi.org/10.55627/agrivet.003.03.0991

Keywords:

Human Recombinant Erythropoietin, Hematobiochemical, Rabbits, Nephrectomy

Abstract

This study was aimed to evaluate the effects of exogenous human recombinant erythropoietin (rHuEPO) on various hemato-biochemical parameters before and after unilateral nephrectomy. A total of 12 adult healthy rabbits were selected and divided into two groups: experimental group A and control group B. The group A (n=6) were treated with rHuEPO @ 1000 IU via subcutaneous route and placebo group B (n=6) without rHuEPO treatment followed by unilateral nephrectomy. Blood sampling was performed three times; 1st   five days before surgery, 2nd; just before surgery and 3rd was performed after 5th day of unilateral nephrectomy. Evaluation of  hematological and biochemical parameters including red blood cells (RBCs), mean corpuscular volumn (MCV) hemoglobin (Hb),total leukocyte count (TLC), packed cell volume (PCV), reticulocyte count (RC) and platelets counts, serum creatinine and blood urea nitrogen (BUN) was done. The data was analyzed statistically by t-test using SPSS version 20. In group A, values of RBCs, MCV, Hb, PCV, RC and platelets count was high after nephrectomy as compared to group B with a significant difference (p≤0.05). Preoperative values of serum creatinine and BUN in group A was declined after 1st injection of rHuEPO at day 5th postoperatively.  At day 10th there was rise in its value but this rise was non-significant (p≥0.05). It is concluded that, rHuEPO increases the process of erthythropoisis. Due to its safety, efficacy and lack of systemic effects, rHuEPO can be used as alternate therapy of blood transfusions and hemato-biochemical disorders while performing nephrectomy or any other surgical interventions.

References

Adamson JW, Eschbach JW and Finch CA. 1968. The kidney and erythropoiesis. The American Journal of Medicine. 44(5):725-33.

Assarasakorn S, Kaewthamasorn M and Niwetpathomwat A. 2008. A retrospective study of clinical use of ecombinant human erythropoietin for treatment of anemia in dogs with renal failure in Bangkok,Thailand. Comp Clin Pathol.2(1):135-143.

Casati S, Passerini P, Campise MR, Graziani G, Cesana B, Perisic M, Ponticelli C. 1987. Benefits and risks of protracted treatment with human recombinant erythropoietin in patients having haemodialysis. Br Med J Clin Res Ed. 295(6605): 1017-1020.

Darby C, Raine AE, Cranston D, Morris PJ. 1993. Effect of prior bilateral nephrectomy on hemoglobin and blood pressure outcome after transplantation. Nephrol Dial Transplant.8(10):1151-1154.

Daniel WW. 2010. Biostatistics: basic concepts and methodology for the health sciences. 9th Ed. John Wiley & Sons, New York. 255-262.

Dong W, Zhang Z, Zhao J, Wu J, Oichai CS, Palacios DA, Antonio EC, Babbar S, Remer EM, Li J, Isharwal S, Zabell J and Campbell SC. 2017. Excised parenchymal mass during partial nephrectomy. J. Urol. 103:129-135.

Eid T. and Brines M. 2002. Recombinant human erythropoietin for neuroprotection: what is the evidence?. Clinical Breast Cancer. 109-115.

Evatt BL, Jerry L. Spivak, and Jack L. 1976. "Relationships between thrombopoiesis and erythropoiesis: with studies of the effects of preparations of thrombopoietin and erythropoietin. J Vet Anim Res. 1(1): 547-558.

Ferraris VA, Ferraris SP, Saha SP, Hessel II EA, Haan CK, Royston BD, Bridges CR, Higgins RS, Despotis. 2007. Clin Pathol. 3: 141-145.

Flaharty KK, Caro J, Erslev A, Whalen JJ, Morris EM, Bjornsson TD, Vlasses PH. 1990. Nephrol. 1(2): 220-225.

Flasher, J. and Drury, D.R. 1949. Effects of removal of the ‘ischemic’kidney in rabbits with unilateral renal hypertension, as compared to unilateral nephrectomy in normal rabbits. American Journal of Physiology-Legacy Content.158(3): 438-443.

Gbrown JR and Spiess BD. 2007. Perioperative blood transfusion and blood conservation in cardiac surgery.Ann Thorac Surg. 83(5): 27-86.

Gieser SM, Fenves AZ. 2006. Chronic kidney disease, dialysis, & transplantation. Proc Bayl Univ MedCent. 19(1): 69-70.

Henke J, Astner S, Brill T, Eissner B, Busch R, Erhardt W. 2005. Comparative study of three intramuscular anaesthetic combinations (medetomidine-ketamine, medetomidine-fentanyl-midazolam and xylazine/ketamine) in rabbits. Veterinary anaesthesia and analgesia. 32(5): 261-70.

Hedley J. 2018. Antibiotic usage in rabbits and rodents. In Practice. 40(6): 230-7.

Inoue N, Takeuchi M, Ohashi H and Suzuki T. 1995. The production of recombinant human erythropoietin. Biotechnol Annu Rev. 1(2): 297-313.

Katsuda Y, Kemmochi Y, Maki M, Sano R, Toriniwa Y, Ishii Y, Miyajima K, Kakimoto K, Ohta, T. 2014. Effects of unilateral nephrectomy on renal function in male Spontaneously Diabetic Torii fatty rats: a novel obese type 2 diabetic model. J Diabetes Res.1(1): 1-6.

Khanam S, Begum N, Hoque AE. 2013. Relationship of Hemoglobin, Packed Cell Volume and Total Count of RBC with the Severity of Chronic Renal Failure. Chat Maa-O-Shis Hosp Med Coll J. 12(2): 31-34.

Latif SM, Khan MA, Mahmood AK, Rashid HB. 2007. Effect of partial and complete nephrectomy on various blood parameters in dogs. J Anim Pl. Sci.17(1-2):1-4.

Mook DM. 2005. The Use of Analgesics in Rodents and Rabbits. Doctoral dissertation, Ph. D., Emory University. 141-149.

Mapara M, Thomas BS, Bhat KM. 2012. Rabbit as an animal model for experimental research. Dent Res J. 9(1): 111-118.

Moore DM, Zimmerman K, Smith SA. 2015. Hematological assessment in pet rabbits: blood sample collection and blood cell identification. Clinics in Laboratory Medicine. 35(3): 617-27.

Pablo LS, Webb AI, Mcnivholas JR. 1995. The effects of atropine and Glycopyrrolate on heart rates in conscious mature goats. Veterinary Surgery. 24(6):531-544.

Perera R, Isola L and Kaufmann H. 1995. Effect of recombinant erythropoietin on anemia and orthostatic hypotension in primary autonomic failure. Clin Auton Res. 5(4): 211-213.

Percy D. 1995. The biology of laboratory rabbit. 2nd ed. Can Vet J.36(10): 646-647.

Phillips CK, Hruby GW, Mirabile G, Motamedinia P, Lehman DS, Okhunov Z, Singh H, Schwartz M, Benson MC and Landman J. 2009. Erythropoietin-induced optimization of renal function after warm ischemia.J Endourol. 23(3): 359-65.

Rhody JL. 2006. Unilateral nephrectomy for hydronephrosis in a pet rabbit. Vet Clin North Am Exot Anim Pract. 9(3): 633-641.

Sabater M, Mancinelli E. and Denk, D. 2021. Renal embryonal nephroma exhibiting malignant features in a Dutch rabbit (Oryctolagus cuniculus). Journal of Exotic Pet Medicine. 37: 18-21.

Sharwood L. 2007.The blood, principles of human physiology.1st Ed.. 464-467. Shimpo H, Mizumoto T, Onoda K, Yuasa H, Yada I (1997). Erythropoietin in pediatric cardiac surgery: clinical efficacy and effective dose. Chest. 111(6): 1565-1570.

Sharples EJ. and Yaqoob, M.M. 2006. Erythropoietin in experimental acute renal failure. Nephron Experimental Nephrology.104(3):83-88.

Stenzel KH, Cheigh JS, Sullivan JF, Tapia L, Riggio RR, Rubin AL. 1975. Clinical effects of bilateral nephrectomy. Am J Med. 58(1): 69-75.

Stohlawetz PJ, Dzirlo L, Hergovich N, Lackner E, Mensik C, Eichler HG, Kabrna E, Geissler K, Jilma B. 2000. Effects of erythropoietin on platelet reactivity and thrombopoiesis in humans. Blood, The Journal of the American Society of Hematology. 95(9): 2983-9.

Slatter DH. 2003. Textbook of Small Animal Surgery. Elsevier Health Sciences (USA). 3rd Ed. 1608-1610.

Szabo Z, Bradley K. and Cahalane, A.K. 2016. Rabbit soft tissue surgery. Veterinary Clinics: Exotic Animal Practice. 19(1): 159-188.

Urie BK, Tillson DM, Smith CM, Brawner WR, Almond GT, Beard DM, Lenz SD, Lothrop CD. 2007. Evaluation of clinical status, renal function, and hematopoietic variables after unilateral nephrectomy in canine kidney donors. Journal of the American Veterinary Medical Association. 1(11): 1653-1656.

White GL. and Holmes DD. 1976. A comparison of ketamine and the combination ketamine-xylazine for effective surgical anesthesia in the rabbit. Laboratory Animal Science. 26(5): 804-806.

Zhao HL, Sui Y, Guan J, He L, Zhu X, Fan RR, Xu G, Alice PS, Ho CS, Fernand MM, Rowlands DK,Juliana CN, and Peter CY. 2008. Fat redistribution and adipocyte transformation in uninephrectomized rats. Kidney Int.;74(4):467-477.

Downloads

Published

2024-12-29

Issue

Vol. 3 No. 3 (2024): J. Agric. Vet. Sci.

Section

Research Articles

License

Copyright (c) 2024 Muhammad Asif, Hamid Akbar, Waqas Haroon, Sana Saeed, Samia Mushtaq, Muhammad Ali, Asma Fatima, Muhammad Zahir, Abdul Aziz, Fazal Rehman

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

The Effects of Human Recombinant Erythropoietin on Various Hemato-biochemical Parameters in Nephrectomized Rabbits (M. Asif, H. Akbar, W. Haroon, S. Saeed, S. Mushtaq, M. Ali, A. Fatima, M. Zahir, A. Aziz, & F. Rehman, Trans.). (2024). Journal of Agriculture and Veterinary Science, 3(3), 521-528. https://doi.org/10.55627/agrivet.003.03.0991

Similar Articles

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)