Assessment of Various Microbiological and Plant-Based Antagonists against Fusarium Wilt Pathogen Fusarium oxysporum.
DOI:
https://doi.org/10.55627/pbulletin.004.01.1207Keywords:
Fusarium oxysporum, microbiological antagonists, plant-based antagonists, sesameAbstract
The wilt disease of sesame (Sesamum indicum L.) caused by Fusarium oxysporum is one of the most significant fungal diseases that affect yield production. In this study, two different approaches, namely in vivo and in vitro, were focused on, in which different microbiological and plant-based antagonists were investigated after 3, 5, and 7 days of incubation to inhibit the development of F. oxysporum in sesame. The results of this study revealed great varietal differences in the growth inhibition of F. oxysporum at all growth stages. In in vitro studies, Bacillus subtilis was more effective in growth inhibition (98.67%) of F. oxysporum among the bacterial antagonists. In fungal antagonists, Aspergillus niger showed a great potential of maximum inhibition in mycelium growth (92.43%) and in plant-based antagonists, Cannabis sativa exhibited maximum antifungal activity (85.5%) as compared to control. However, the highest bacterial antagonistic activity was evaluated comparatively to fungal and plant-based antagonists. Whereas, in vivo, the study showed the highest antifungal activity (38% - 69%) of selected fungal antagonists, followed by plant-based antagonists (34.17% - 65%) and bacterial antagonists (20% - 48%). This study would help the agrochemical companies to develop microbial and plant-based fungicides that are non-phytotoxic and eco-friendly for the management of fungal diseases, as compared to chemical fungicides that are harmful to consumers and pollute the environment.
References
REFERENCES
Adhikari, M., Kharel, N., Gaire, L., Poudel, R., Shrestha, S. M., Gaire, S. P., & Acharya, B. (2018). In vitro evaluation of different chemicals against Rhizoctonia solani by poisoned food technique. Field Crop, 1.
Alkooranee, J. T., Al-khshemawee, H. H., Al-badri, M. A. K., Al-srai, M. S., & Daweri, H. H. (2020). Antifungal activity and GC-MS detection of leaves and root parts of Chenopodium album extract against some phytopathogenic fungi. Indian Journal of Agricultural Research, 54(1), 117-121. http://dx.doi.org/10.18805/IJARe.A-433
Alqurashy, N. N. A. (2017). Antibacterial and antifungal activity of the extract of medicinal plant Malva neglecta Wallr. Wasit Journal for Science & Medicine, 10(1), 1-6. http://dx.doi.org/10.31185/jwsm.407
Ara, A.; A Akram; M. Ajmal; S. Akhund; B. G. Nayyar; W. Seerat and S. M. Chaudhry (2017). Histopathological studies of sesame (Sesamum indicum) seedlings infected with Fusarium oxysporum. Plant Pathology & Quarantine, (7)1: 82–90. http://dx.doi.org/10.5943/ppq/7/1/10
Azwanida, N. N. "A review on the extraction methods use in medicinal plants, principle, strength and limitation." Med aromat plants 4.196 (2015): 2167-0412. http://dx.doi.org/10.4172/2167-0412.1000196
Belay, Y. (2018). Screening of fusarium wilt, bacterial blight, and phyllody diseases resistant sesame genotypes in sesame growing areas of northern Ethiopia. J. Agric. Ecol. Res. Int, 15(2), 1-12. http://dx.doi.org/10.9734/JAERI/2018/41414
Egonyu, J. P., Kyamanywa, S., Anyanga, W., & Ssekabembe, C. K. (2005). Review of pests and diseases of sesame in Uganda. In African crop science conference proceedings (Vol. 7, No. pt. 03 of 03, pp. 1411-1416). https://api.semanticscholar.org/CorpusID:131315639
Gajbhiye, A., Rai, A. R., Meshram, S. U., & Dongre, A. B. (2010). Isolation, evaluation, and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum. World Journal of Microbiology and Biotechnology, 26, 1187-1194. http://dx.doi.org/10.1007/s11274-009-0287-9
Haas, D. and G. Defago (2005). Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat. Rev. Microbiol., 3 (4): 307–319. https://doi.org/10.1038/nrmicro1129
Hassan, M. A., El-Saadony, M. T., Mostafa, N. G., El-Tahan, A. M., Mesiha, P. K., El-Saadony, F. M., ... & Ashry, N. M. (2021). The use of previous crops as sustainable and eco-friendly management to fight Fusarium oxysporum in sesame plants. Saudi Journal of Biological Sciences, 28(10), 5849-5859. .
Javaid, S., Shah, M. H., Khan, I. H., Javaid, A., & Syed, M. W. (2020). USING EXTRACTS OF LAMBSQUARTERS WEED FOR CONTROL OF Alternaria alternata. Pakistan Journal of Weed Science Research, 26(2), 147 http://dx.doi.org/10.28941/pjwsr.v26i2.824
Lubaina, A.S. and K. Murugan (2015). Evaluation of biological efficacy of Trichoderma species isolates against Alternaria leaf spot disease of sesame. Int. J. Appl. Biol. and Pharm. Technol., 6 (1): 17-24.
Mahmoud, A. F., & Abdalla, O. A. (2018). Biocontrol efficacy of Trichoderma spp. against sesame wilt caused by Fusarium oxysporum f. sp. sesami. Archives of Phytopathology and Plant Protection, 51(5-6), 277-287. http://dx.doi.org/10.1080/03235408.2018.1471837
Mahmoud, S. Y., Ziedan, E. S. H., Farrag, E. S., Kalafalla, R. S., & Ali, A. M. (2016). Antifungal activity of pyocyanin produced by Pseudomonas aeruginosa against Fusarium oxysporum Schlech phytopathogenic fungi. Int J PharmTech Res, 9, 43- 50.
Mohiddin, F.A., Khan, M.R., Khan, S.M. and Bhat, B.H. (2010). Why Trichoderma is considered a superhero (super fungus) against the evil parasites. Plant Pathol. J., 9: 92-102. http://dx.doi.org/10.3923/ppj.2010.92.102
Naher, L., Yusuf, U. K., Ismail, A., & Hossain, K. (2014). Trichoderma spp.: a biocontrol agent for sustainable management of plant diseases. Pak. J. Bot, 46(4), 1489-1493.
Nayyar, B. G., Woodward, S., Mur, L. A., Akram, A., Arshad, M., Naqvi, S. S., & Akhund, S. (2018). Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.) seeds from the Punjab, Pakistan. Physiological and molecular plant pathology, 102, 128-135. https://doi.org/10.1016/j.pmpp.2018.02.001
Ojiambo, P. S., Mibey, R. K., Narla, R. D., & Ayiecho, P. O. (2003). Field transmission efficiency of Alternaria sesami in sesame from infected seed. Crop Protection, 22(9), 1107-1115. https://doi.org/10.1016/S0261-2194(03)00144-3
Pandey, S., & Gupta, R. (2014). Screening of nutritional, phytochemical, antioxidant, and antibacterial activity of Chenopodium album (Bathua). Journal of Pharmacognosy and Phytochemistry, 3, 01-09.
Patel, A. B., & Bhattacharya, P. (2021). Challenges and prospects of managing charcoal rot in sesame with chemical fungicides: A review. Journal of Plant Diseases and Protection, 128(2), 391-399.
Rauf, S., & Javaid, A. (2013). Antifungal activity of different extracts of Chenopodium album against Fusarium oxysporum f. sp. cepae, the cause of onion basal rot. International Journal of Agriculture and Biology, 15(2). https://www.webofscience.com/wos/WOSCC/full-record/000319659600030
Swain, S., Barik, S. K., Behera, T., Nayak, S. K., Sahoo, S. K., Mishra, S. S., & Swain, P. (2016). Green synthesis of gold nanoparticles using root and leaf extracts of Vetiveria zizanioides and Cannabis sativa and its antifungal activities. BioNanoScience, 6, 205-213. https://link.springer.com/article/10.1007/s12668-016-0208-y
Sharma, B. K., Loganathan, M., Singh, R. P., Bag, T. K., Rai, R. K., Rai, A. B., & Rai, M. (2011). Aspergillus niger, a potential biocontrol agent for controlling fusarial wilt of tomato. http://www.imskolkata.org/
Singh, P., Pandit, S., Garnæs, J., Tunjic, S., Mokkapati, V. R., Sultan, A.,& Mijakovic, I. (2018). Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. International journal of nanomedicine, 3571-3591. https://doi.org/10.2147/ijn.s157958
Zulqarnain, Iqbal, Z., Cox, R., Anwar, J., Ahmad, N., Khan, K., & Khattak, S. U. (2020). Antifungal activity of compounds isolated from Aspergillus niger and their molecular docking studies with tomatinase. Natural product research, 34(18), 2642-2646. https://doi.org/10.1080/14786419.2018.1548447
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