Morphological and Fatty Acid Diversity in Camelina sativa Mutant Lines for Low-Input Biodiesel Production in Sub-Tropical Environments
DOI:
https://doi.org/10.55627/pbulletin.004.02.1451Keywords:
Camelina sativa; Mutant germplasm; Fatty acid composition; Low-input agriculture; Biodiesel feedstock; Climate-resilient cropsAbstract
Camelina sativa has emerged as a promising oilseed crop for biodiesel and ethanol production due to its high yield potential under minimal input conditions, making it suitable for marginal and rainfed lands. In this study, a three-year field evaluation of 50 mutant lines was conducted under sub-tropical low-input conditions to assess morphological variation and oil quality. Significant genetic variability was observed across key agronomic traits such as plant height (48–78 cm), capsule number (338–5627), and seed yield (6–14 g per plant), indicating ample scope for selection. Fatty acid profiling revealed considerable variation in oleic acid (0–26.5%) and palmitoleic acid (0–63.6%), with several lines rich in unsaturated fatty acids, essential for biodiesel quality and oxidative stability. Notably, mutant lines 6783, 4844, 5958, 5989, and 6725 exhibited superior yield performance, while lines such as 6862, 6882, 5296, and 6795 showed desirable oil profiles for biofuel. These findings underline the potential of selected mutant lines to serve as climate-resilient, low-input feedstock for sustainable biodiesel production. This work contributes toward the development of environment-friendly cropping systems and energy diversification strategies aligned with climate adaptation and SDG 7 (Affordable and Clean Energy) and SDG 13 (Climate Action) goals.
References
Abdullah, H. M., Pang, N., Chilcoat, B., Shachar-Hill, Y., Schnell, D. J., & Dhankher, O. P. (2024). Overexpression of the phosphatidyl choline: diacylglycerol choline phosphotransferase (PDCT) gene increases carbon flux toward triacylglycerol (TAG) synthesis in Camelina sativa seeds. Plant Physiology and Biochemistry, 208, 108470. https://doi.org/10.1016/j.plaphy.2024.108470
Ahmad, M., et al. (2022). Changing climate scenario: Perspectives of Camelina sativa as low-input biofuel and oilseed crop. In M. Ahmed (Ed.), Global agricultural production: Resilience to climate change (pp. 197–236). Springer. https://doi.org/10.1007/978-3-031-14973-3_7
Altin, R., Selim, C., & Huseyin, S. Y. (2001). The potential of using vegetable oil fuels as fuel for diesel engines. Energy Conversion and Management, 42(5), 529–538. https://doi.org/10.1016/S0196-8904(00)00078-0
Angelini, L. G., Abou Chehade, L., Foschi, L., &Tavarini, S. (2020). Performance and potentiality of Camelina sativa genotypes in response to sowing date under Mediterranean environment. Agronomy, 10(12), 1929. https://doi.org/10.3390/agronomy10121929
AOAC International. (2000). Official methods of analysis of AOAC International (17th ed.). Gaithersburg, MD, USA: AOAC International.
Aslam, M. M., Khan, A. A., Cheema, H. M. N., Hanif, M. A., Azeem, M. W., & Azmat, M. A. (2020). Novel mutant Camelina and Jatropha as valuable feedstocks for biodiesel production. Scientific Reports, 10, 21868. https://doi.org/10.1038/s41598-020-78927-z
Bachlava, E., Burton, J. W., Brownie, C., Wang, S. B., Auclair, J., & Cardinal, A. J. (2008). Heritability of oleic acid content in soybean seed oil and its genetic correlation with fatty acid and agronomic traits. Crop Science, 48, 1764–1773. https://doi.org/10.2135/cropsci2007.09.0512
Bang, G. (2010). Energy security and climate change concerns: Triggers for energy policy change in the United States? Energy Policy, 38(4), 1645–1653. https://doi.org/10.1016/j.enpol.2009.01.045
Bernardo, A., Howard-Hildige, R., O'Connell, A., Nichol, R., Ryan, J., Rice, B., Roche, E., & Leahy, J. J. (2003). Camelina oil as a fuel for diesel transport engines. Industrial Crops and Products, 17(3), 191–197. https://doi.org/10.1016/S0926-6690(02)00159-3
Berti, M., Wilckens, R., Fischer, S., Solis, A., & Johnson, B. (2011). Seeding date influence on Camelina yield, yield components, and oil content in Chile. Industrial Crops and Products, 34(3), 1358–1365. https://doi.org/10.1016/j.indcrop.2010.12.006
Cai, Y., Liang, Y., Shi, H., Cui, J., Prakash, S., Zhang, J., Anaokar, S., Chai, J., Schwender, J., Lu, C., Yu, X.-H., & Shanklin, J. (2024). Creating yellow seed Camelina sativa with enhanced oil accumulation by CRISPR‐mediated disruption of Transparent Testa 8. Plant Biotechnology Journal, 22(10), 2773–2784. https://doi.org/10.1111/pbi.14201
Clark, C. (2011). Colorado agricultural statistics. National Agricultural Statistics Service.
Clavijo-Bernal, E. J., Martínez-Force, E., Garcés, R., Salas, J. J., & Venegas-Calerón, M. (2024). Biotechnological Camelina platform for green sustainable oleochemicals production. OCL, 31, 11. https://doi.org/10.1051/ocl/2024006
Ebrahimi, A., Chenar, H. M., Rashidi-Monfared, S., & Kahrizi, D. (2025). Enhancing food security via selecting superior Camelina sativa parents: A positive approach incorporating pheno-morphological traits, fatty acid composition, and tocopherols content. BMC Plant Biology, 25, 53. https://doi.org/10.1186/s12870-024-04567-1
Ehrensing, D. T., & Guy, S. O. (2008). Oilseed crops: Camelina (EM 8953-E). Oregon State University Extension Service.
Francis, C. M., & Campbell, M. C. (2003). New high quality oil seed crops for temperate and tropical Australia. Rural Industries Research and Development Corporation.
Gabriel, K. R. (1981). Biplot display of multivariate matrices for inspection of data and diagnosis. In V. Barnett (Ed.), Interpreting multivariate data (pp. 147–173). John Wiley & Sons.
Gauraha, D., & Rao, S. S. (2011). Association analysis for yield and its characters in linseed (Linum usitatissimum L.). Research Journal of Agricultural Sciences, 2, 258–260.
Gesch, R. W., & Cermak, S. C. (2011). Sowing date and tillage effects on fall-seeded camelina in the northern Corn Belt. Agronomy Journal, 103(4), 980–987. https://doi.org/10.2134/agronj2010.0414
Ghidoli, M., Ponzoni, E., Araniti, F., Miglio, D., & Pilu, R. (2023). Genetic Improvement of Camelina sativa (L.) Crantz: Opportunities and Challenges. Plants, 12(3), Article 570.
Gugel, R. K., & Falk, K. C. (2006). Agronomic and seed quality evaluation of Camelina sativa in western Canada. Canadian Journal of Plant Science, 86(4), 1047–1057. https://doi.org/10.4141/P05-169
Hunsaker, D. J., French, A. N., & Thorp, K. R. (2012). Camelina water use and seed yield response to irrigation scheduling in an arid environment. Irrigation Science. https://doi.org/10.1007/s00271-012-0368-7
Iskandarov, U., Kim, H. J., & Cahoon, E. B. (2014). Camelina: An emerging oilseed platform for advanced biofuels and bio-based materials. In Plants and bioenergy (pp. 131–140). Springer.
Jeffers, J. N. R. (1967). Two case studies in the application of principal component analysis. Journal of the Royal Statistical Society: Series C (Applied Statistics), 16(3), 225–236. https://doi.org/10.2307/2985936
Jiang, Y., Caldwell, C. D., & Falk, K. (2014). Camelina seed quality in response to applied nitrogen, genotype and environment. Canadian Journal of Plant Science, 94(6), 971–980. https://doi.org/10.4141/cjps2014-052
Johnson, D. L. (2006). Introduction and production of Camelina. Montana State University, Northwest Agricultural Research Center.
Katar, D., Arslan, Y., & Subasi, I. (2012). Genotypic variation on yield, yield components and oil quality in some camelina genotypes. Turkish Journal of Field Crops, 17(2), 105–110.
Kirkhus, B., Lundon, A. R., Haugen, J.-E., Vogt, G., Borge, G. I. A., & Henriksen, B. I. (2013). Effects of environmental factors on edible oil quality of organically grown Camelina sativa .Journal of Agricultural and Food Chemistry, 61, 3179–3185. https://doi.org/10.1021/jf303994z
Kjeldahl, J. (1883). New method for the determination of nitrogen in organic substances. Journal of Analytical Chemistry, 22(1), 366–382.
Kovach Computing Services. (n.d.). MVSP – Multivariate Statistical Package (Version 3.1). https://mvsp.software.informer.com/3.1/
Kwon, S. H., & Torrie, J. H. (1964). Heritability and interrelationship among traits of two soybean populations. Crop Science, 4(2), 196–198.
Lothorp, J. E., Akins, R. E., & Smith, O. S. (1985). Variability of yield and yield components in IAPIR grain sorghum random mating population; means, variance components and heritabilities. Crop Science, 25, 235–240.
Lovett, J. V., & Duffield, A. M. (1981). Allelochemicals of Camelina sativa .Journal of Applied Biology, 18(1), 283–290.
McVay, K. A., & Lamb, P. F. L. (2008). Camelina production in Montana. Montguide, Montana State University Extension.
Muduli, K. C., & Patnaik, M. C. (1994). Character association and path coefficient analysis in linseed (Linum usitatissimum L.). Orissa Journal of Agricultural Research, 7, 5–7.
Pavlista, A. D., & Baltensperger, D. D. (2007). Phenology of oilseed crops for bio-diesel in the high plains. In J. Janick & A. Whipkey (Eds.), Issues in new crops and new uses (pp. 60–63). ASHS Press.
Pilgeram, A. L., Sands, D. C., Boss, D., Dale, N., Wichman, D., Lamb, P., Lu, C., Barrows, R., Kirkpatrick, M., Thompson, B., & Johnson, D. L. (2007). Camelina sativa , a Montana omega-3 and fuel crop. In J. Janick & A. Whipkey (Eds.), Issues in new crops and new uses (pp. 129–131). Alexandria, VA: ASHS Press.
Poehlman, J. M., & Sleeper, D. A. (1995). Breeding field crops (4th ed., pp. 75–76). Panima Publishing.
Sharma, S., Bajaj, R. K., Kaur, N., & Sehgal, S. K. (2003). Combining ability studies in sunflower (Helianthus annuus L.). Crop Improvement, 30(1), 69–73.
Soxhlet, H. (1879). Preparation of permanent rennet-essence. American Journal of Pharmacy (1835–1907), 51, 37.
Steel, R. D. G., Torrie, J. H., & Dickey, D. A. (1997). Principles and procedures of statistics: A biometrical approach (3rd ed.). McGraw Hill.
Tadesse, T., Singh, H. A. R. J. I. T., & Weyessa, B. (2009). Correlation and path coefficient analysis among seed yield traits and oil content in Ethiopian linseed germplasm. International Journal of Sustainable Crop Production, 4(4), 8–16.
Tukey, J. W. (1949). Comparing individual means in the analysis of variance. Biometrics, 5(2), 99–114.
United Nations. (2015). Transforming our world: The 2030 agenda for sustainable development. https://sdgs.un.org/2030agenda
USDA. (2010). Biofuels strategic production report. U.S. Department of Agriculture.
Van Belle, J., Schaart, J. G., Dechesne, A. C., Fei, D., Cisneros, A. O., Serafini, M., ... & van Loo, E. N. (2025). Direct and indirect effects of multiplex genome editing of F5H and FAD2 in oil crop camelina. Plant Biotechnology Journal, 23(5), 1399–1412. https://doi.org/10.1111/pbi.14120
Vollmann, J., Damboeck, A., Eckl, A., Schrems, H., &Ruckenbauer, P. (1996). Improvement of Camelina sativa , an underexploited oilseed. In Progress in new crops: Proceedings of the Third National Symposium (pp. 357–362). ASHS Press.
Vollmann, J., Grausgruber, H., Stift, G., Dryzhyruk, V., & Lelly, T. (2005). Genetic diversity in camelina germplasm as revealed by seed quality characteristics and RAPD polymorphism. Plant Breeding, 124, 446–453. https://doi.org/10.1111/j.1439-0523.2005.01123.x
Weather Atlas. (2024). Faisalabad climate: Weather by month, temperature, rainfall, etc.https://www.weather-atlas.com/en/pakistan/faisalabad-climate
Yan, W., & Kang, M. S. (2011). GGE biplot analysis: A graphical tool for breeders, geneticists, and agronomists. CRC Press.
Zanetti, F., Eynck, C., Christou, M., Krzyżaniak, M., Righini, D., Alexopoulou, E., Stolarski, M. J., van Loo, E. N., Puttick, D., & Monti, A. (2017). Agronomic performance and seed quality attributes of Camelina sativa in multi-environment trials across Europe and Canada. Industrial Crops and Products, 107, 602–608. https://doi.org/10.1016/j.indcrop.2017.06.022
Zubr, J. (2003). Qualitative variation of Camelina sativa seed from different locations. Industrial Crops and Products, 17(3), 161–169. https://doi.org/10.1016/S0926-6690(02)00156-8
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Muhammad Abubakkar Azmat, Asif Ali Khan, Muhammad Mahran Aslam, Saman Arshad, HUMERA RAZZAQ

This work is licensed under a Creative Commons Attribution 4.0 International License.
