Impact of heat stress, molecular responses, and breeding strategies for heat tolerance in cotton (Gossypium hirsutum L.)

Authors

  • Iqra Parveen Cotton Research Station, Ayub Agricultural Research Institute, Faisalabad, Pakistan
  • Jehanzeb Farooq Cotton Research Station, Ayub Agricultural Research Institute, Faisalabad, Pakistan
  • Muhammad Safyan Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
  • Farrukh Ellahi Cotton Research Station, Ayub Agricultural Research Institute, Faisalabad, Pakistan.
  • Amna Nazir Cotton Research Station, Ayub Agricultural Research Institute, Faisalabad, Pakistan
  • Ghulam Sarwar Cotton Research Institute, Multan, Pakistan
  • Wajeeha khan ayub agricultural research institute , Vegetable Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan.

DOI:

https://doi.org/10.55627/zoobotanica.003.02.1197

Keywords:

Cotton, high temperature, impact, mechanisms, strategies

Abstract

Cotton is a vital cash crop in many countries due to its multipurpose usage and value addition to Gross Domestic Production (GDP), but due to climate change, it is extremely vulnerable to biotic and abiotic stresses. Among abiotic stresses, heat stress (HS) has emerged as a critical environmental constraint affecting cotton productivity and fiber quality, particularly under the escalating influence of global climate change. HS affects the cotton plant at morphological, physiological and molecular levels such as activation of various microRNAs (miRNAs), reactive oxygen species (ROS), signaling pathways including calcium, kinases, carbohydrates, gene expression regulation, epigenetic regulation, and plant hormones that help mitigate cellular damage. The purpose of this manuscript is to study the impacts of HS at critical stages of plant growth by thorough understanding of underlying mechanisms and regulatory pathways and explore the genetic underpinnings of cotton to aid breeders in employing various strategies such as conventional breeding, molecular and transgenic approaches to enhance thermal resilience. A deeper understanding of these adaptive responses is essential for sustaining cotton production in increasingly warmer climates.

References

Anjum, Z. I., Azhar, M. T., Hayat, K., Ashraf, F., Shahzad, U., & Azam, M. 2014. Development of high yielding and CLCuV resistant ulpand cotton variety “CIM-608”. Pakistan Journal of Phytopathology. 26(1):25-34.

Abro, S., Rajput, M. T., Khan, M. A., Sial, M. A., & Tahir, S. S. 2015. Screening of cotton (Gossypium hirsutum L.) genotypes for heat tolerance. Pakistan Journal of Botany 47(6):2085-2091.

Ahsan, M. Z., Khan, M. I., Bhutto, H., Anjum, R., Majidano, M. S., Bano, S., Soomro, A. W., Panhwar, F. H., Channa, A. R., & Malik, T. H. 2017. Registration of ‘CRIS-129’, an early-maturing, heat-tolerant, and high-yielding cotton cultivar. Journal of Plant Registrations 11(3):222-227. https://doi.org/10.3198/jpr2016.07.0037crc.

Abro, S., Rizwan, M., Deho, Z. A., Abro, S. A., & Sial, M. A. 2022. Identification of heat tolerant cotton lines showing genetic variation in cell membrane thermostability, stomata, and trichome size and its effect on yield and fiber quality traits. Frontiers in Plant Science 12:1-15.

Ali, M. M., Ali, Z., Ahmad, F., Nawaz, F., Shakil, Q., Ahmad, S., & Khan, A. A. 2022. Transcript abundance of heat shock protein genes confer heat tolerance in cotton (Gossypium hirsutum L.). Pakistan Journal of Botany 54(1):65-71.

Anwar, M., Saleem, M. A., Dan, M., Malik, W., Ul-Allah, S., Ahmad, M. Q, Qayyum, A., Amjid, M. W., Zia, Z. U., Afzal, H., Asif, M., Rahman, M. A., & Hu, Z. 2022. Morphological, physiological and molecular assessment of cotton for drought tolerance under field conditions. Saudi journal of biological sciences. 29(1):444-452.

Abro, A. A., Anwar, M., Javwad, M. U., Zhang, M., Liu, F., Jiménez-Ballesta, R., Salama, E. A. A., & Ahmed, M. A. A. 2023. Morphological and physio-biochemical responses under heat stress in cotton: Overview. Biotechnology Reports 40:1-9. https://doi.org/10.1016/j.btre.2023.e00813.

Ali, Z., Maryam, H., Saddique, M. A. B., & Ikram, R. M. 2023. Exploiting genetic diversity in enhancing phenotypic plasticity to develop climate-resilient cotton. Genetic Resources and Crop Evolution 70:1305-1320. doi:10.1007/s10722-023-01554-3

Adjibolosoo, D., Nartey, M. A., Aboagye, E. T., Danso, B., Fatima, I., Hua, J., & Guo, A. 2024. Genome-wide studies and expression profiling of GhWRKY41 family genes in different tissues and stress conditions in upland cotton (Gossypium hirsutum). Industrial Crops and Products. 215: 118486. doi: 10.1016/j.indcrop.2024.118486

Ahmed, S. R., Asghar, M. J., Hameed, A., Ghaffar, M., & Shahid, M. 2024. Advancing crop improvement through GWAS and beyond in mung bean. Frontiers in Plant Science. 15: 1-28.

Ashraf, S., Ch, K. M., Ashraf, I., & Akbar, N. 2024. A phenomenological inquiry into farmers’ experiences growing cotton in Punjab, Pakistan. Scientific reports. 14(1): 13846.

Altaf, M. T., Cavagnaro, P. F., Kökten, K., Ali, A., Morales, A., Tatar, M., Bedir, M., Nadeem, M. A., Aasim, M., Celiktas, N., Mansoor, S., & Baloch, F. S. 2025. Genotyping-by-sequencing derived SNP markers reveal genetic diversity and population structure of Dactylis glomerata germplasm. Frontiers in Plant Science. 16: 1-15.

Bo, X., Zhou, Z. G., Guo, L. T., Xu, W. Z., Zhao, W. Q., Chen, B. L., Meng, Y. L., & Wang, Y. H. 2017. Susceptible time window and endurable duration of cotton fiber development to high temperature stress. Journal of Integrative Agriculture 16(9):1936-1945.

Batcho, A. A., Sarwar, M. B., Rashid, B., Hassan, S., & Husnain, T. 2021. Heat shock protein gene identified from Agave sisalana (As HSP70) confers heat stress tolerance in transgenic cotton (Gossypium hirsutum L.). Theoretical and Experimental Plant Physiology 33(2):141-156. https://doi.org/10.1007/s40626-021-00200-6.

Billings, G. T., Jones, M. A., Rustgi, S., Bridges, W. C, Holland, J. B., Hulse-Kemp, A. M., & Campbell, B. T. 2022. Outlook for Implementation of genomics-based selection in public cotton breeding programs. Plants. 11(11):1-16.

Considine, M. J., Sandalio, L. M., & Foyer CH. 2015. Unravelling how plants benefit from ROS and NO reactions, while resisting oxidative stress. Annals of Botany. 116(4):469-473. https://doi.org/10.1093/aob/mcv153.

Chen, E., Wang, X., Gong, Q., Butt, H. I., Chen, Y., Zhang, C., Yang, Z., Wu, Z., Ge, X., Zhang, X., Li, F., & Zhang X. 2017. A novel GhBEE1-like gene of cotton causes anther indehiscence in transgenic Arabidopsis under uncontrolled transcription level. Gene 627: 49-56.

Chen, J., Su, P., Chen, P., Li, Q., Yuan, X., & Liu Z. 2018. Insights into the cotton anther development through association analysis of transcriptomic and small RNA sequencing. BMC plant biology 18: 1-12.

Chen, G., Li, J., Liu, Y., Zhang, Q., Gao, Y., Fang, K., Cao, Q., Qin, L., & Xing Y. 2019. Roles of the GA-mediated SPL gene family and miR156 in the floral development of Chinese chestnut (Castanea mollissima). International Journal of Molecular Sciences. 20(7):1-17. https://doi.org/10.3390/ijms20071577.

Dabbert, T. A. 2014. Genetic analysis of cotton evaluated under high temperature and water deficit (PhD thesis: The University of Arizona).

Ding, Y., Ma, Y., Liu, N., Xu, J., Hu, Q., Li, Y., Wu, Y., Xie, S., Zhu, L., Min, L., & Zhang, X. 2017. microRNAs involved in auxin signalling modulate male sterility under high‐temperature stress in cotton (Gossypium hirsutum). The Plant Journal. 91(6): 977-994.

Ding, B., Liu, B., Zhu, X., Zhang, H., Hu, R., Li, S., Zhang, L., Jiang, L., Yang, Y., Zhang, M., Zhao, J., Pei, Y., & Hou, L. 2024. Downregulation of the GhROD1 gene improves cotton fiber fineness by decreasing acyl pool saturation, stimulating small heat shock proteins (sHSPs), and reducing H2O2 production. International Journal of Molecular Sciences. 25: 1-14.

Echer, F. R., Oosterhuis, D. M., Loka, D. A., & Rosolem CA. 2014. High night temperatures during the floral bud stage increase the abscission of reproductive structures in cotton. Journal of Agronony and Crop Science. 200(3):191-198. https://doi.org/10.1111/jac.12056

Ekinci, R., Basbag, S., Karademir, E., & Karademir C. 2017. The effects of high temperature stress on some agronomic characters in cotton. Pakistan Journal of Botany. 49(2):503-508.

Esmaeili, N., Cai, Y., Tang, F., Zhu, X., Smith, J., Mishra, N., Hequet, E., Ritchie, G., Jones, D., & Shen, G. 2021. Towards doubling fiber yield for cotton in the semiarid agricultural area by increasing tolerance to drought, heat and salinity simultaneously. Plant Biotechnology Journal. 19(3):462-476. https://doi.org/10.1111/pbi.13476.

Farooq, J., Khalid, M., Muhammad, W. A., Atiq, R. M., Imran, J., Valentin, P. M. I., & Nawaz N. 2015. High temperature stress in cotton Gossypium hirsutum L. ELBA-Bioflux. 7(1):34-44.

Fahad, S., Bajwa, A. A., Nazir, U., Anjum, S. A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S., Ihsan, M. Z., Alharby, H., Wu, C., Wang, D., & Huang J. 2017. Crop production under drought and heat stress: plant responses and management options. Frontiers in Plant Sciences 8:1-16. https://doi.org/10.3389/fpls.2017.01147.

Gesteiro, N., Malvar, R. A., Butrón, A., Holland, J. B., Souto, X. C., López-Malvar, A., & Santiago, R. 2025. Genome-Wide Association Study and Genomic Predictions for Hydroxycinnamate Concentrations in Maize Stover. Journal of agricultural and food chemistry 73: 2289-2298.

Hatfield, J. L., & Prueger, J. H. 2015. Temperature extremes: Effect on plant growth and development. Weather and Climate Extremes 10:4-10. https://doi.org/10.1016/j.wace.2015.08.001.

He, P., Yang, Y., Wang, Z., Zhao, P., Yuan, Y., Zhang, L., Ma, Y., Pang, C., Yu, J., & Xiao, G. 2019. Comprehensive analyses of ZFP gene family and characterization of expression profiles during plant hormone response in cotton. BMC Plant Biology 19: 1-13.

Han, X., Gao, C., Liu, L., Zhang, Y., Jin, Y., Yan, Q., Yang, L., Li, F., & Yang, Z. 2022. Integration of eQTL analysis and GWAS highlights regulation networks in cotton under stress condition. International Journal of Molecular Sciences 23(14): 1-17.

Hao, X., & He, S. 2024. Genome-wide identification, classification and expression analysis of the heat shock transcription factor family in Garlic (Allium sativum L.). BMC Plant Biology 24: 1-18.

Ijaz, A., Anwar, Z., Ali, A., Ditta, A., Shani, M. Y., Haidar, S., Wang, B., Fang, L., Khan, S. M. D., & Khan, M. K. R. 2024. Unraveling the genetic and molecular basis of heat stress in cotton. Frontiers in Genetics 15:1-15.

Islam, W., Adnan, M., Alomran, M. M., Qasim, M., Waheed, A., Alshaharni, M. O., & Zeng, F. 2024. Plant responses to temperature stress modulated by microRNAs. Physiologia Plantarum. 176(1): e14126.

Jiang, J., Ren, J., Zeng, Y., Xu, X., Lin, S., Fan, Z, Meng, Y., Ma, Y., Li, X., & Wu, P. 2025. Integration of GWAS models and GS reveals the genetic architecture of ear shank in maize. Gene. 938,149140.

Kamal, M., Saleem, M., Shahid, M., Awais, M., Khan, H., & Ahmed, K. 2017a. Ascorbic acid triggered physiochemical transformations at different phenological stages of heat-stressed BT cotton. Journal of Agronomy and Crop Science. 203(4):323-331. https://doi.org/10.1111/jac.12211.

Kamal, M., Saleem, M., Wahid, M., & Shakeel, A. 2017b. Effects of ascorbic acid on membrane stability and yield of heat stressed BT cotton. Journal of Animal and Plant Sciences. 27(1):192-199.

Karademir, E., Karademir, C., Sevilmis, U., & Basal, H. 2018. Correlations between canopy temperature, chlorophyll content and yield in heat tolerant cotton (Gossypium hirsutum L.) genotypes. Fresenius Environmental Bulletin. 27(8):5230-5237.

Khan Z, Khan SH, Ahmed A, Iqbal, M. U., Mubarik, M. S., Ghouri, M. Z., Ahmad, F., Yaseen, S., Ali, Z., Khan, A. A., & Azhar, M. T. 2023. Genome editing in cotton: challenges and opportunities. Journal of Cotton Research. 6(3):1-21.

Liu, N., Tu, L., Wang, L., Hu, H., Xu, J., & Zhang, X. 2017. MicroRNA 157-targeted SPL genes regulate floral organ size and ovule production in cotton. BMC Plant Biology. 17(7):1-14. https://doi.org/10.1186/s12870-016-0969-z.

Lubkowska, A., Pluta, W., Strońska, A., & Lalko, A. 2021. Role of heat shock proteins (HSP70 and HSP90) in viral infection. International journal of molecular sciences. 22(17): 1-17.

Li, Y., Chen, M., Khan, A. H., Ma, Y., He, X., Yang, J., Zhang, R., Ma, H., Zuo, C., Li, Y., Kong, J., Wang, M., Zhu, L., Zhang, X., & Min, L. 2023a. Histone H3 lysine 27 trimethylation suppresses jasmonate biosynthesis and signaling to affect male fertility under high temperature in cotton. Plant Communications. 4(6):1-17.

Li, H., Feng, B., Li, J., Fu, W., Wang, W., Chen, T., Liu, L., Wu, Z., Peng, S., Tao, L., & Fu, G. 2023b. RGA1 alleviates lowlight- repressed pollen tube elongation by improving the metabolism and allocation of sugars and energy. Plant. Cell & Environment. 46: 1363-1383. doi:10.1111/pce.14547

Luo, J., Li, M., Ju, J., Hai, H., Wei, W., Ling, P, Li, D., Su, J., Zhang, X., & Wang, C. 2024. Genome-wide identification of the GhANN Gene Family and functional validation of GhANN11 and GhANN4 under Abiotic Stress. International Journal of Molecular Sciences. 25: 1-22.

Li, Y., Fu, Y., Li, Y., Zhang, R., Yang, J., Ma, H, Min, L., & Zhang, X. 2025. Reversing anther thermotolerance by manipulating the cis-elements in the promoter of a high-temperature upregulated gene Casein Kinase I in upland cotton. Science China Life Sciences. 68: 1558-1569.

Lv, W. B., Miao, C. C., Du, C. H., Cui, Y. T., Liu, M., Shen, M. C., Owusu, A. G., Guo, N., Li, D. H., & Gao, J. S. 2024. CDPK protein in cotton: genomic-wide identification, expression analysis, and conferring resistance to heat stress. BMC Plant Biology. 24: 1-21.

Luqman, T., Hussain, M., Ahmed, S. R., Ijaz, I., Maryum, Z., Nadeem, S., Khan, Z., Khan, S. M. U. D., Aslam, M., Liu, Y., & Khan, M. K. R. 2025. Cotton under heat stress: a comprehensive review of molecular breeding, genomics, and multi-omics strategies. Frontiers in Genetics. 16: 1-30.

Lawson, T. O., Selva, J. P., Carballo, J., Caccamo, M., Sargent, D. J., & Šurbanovski, N. 2025. Developmental processes in the Rosaceae through the lens of DNA and RNA methylation. Planta. 261(3): 1-21.

Min, L., Li, Y., Hu, Q., Zhu, L., Gao, W., Wu, Y., Ding, Y., Liu, S., Yang, X., & Zhang, X. 2014. Sugar and auxin signaling pathways respond to high-temperature stress during anther development as revealed by transcript profiling analysis in cotton. Plant physiology. 164(3):1293-1308.

Mishra, R. C., & Grover, A. 2016. ClpB/Hsp100 proteins and heat stress tolerance in plants. Critical Reviews in Biotechnology. 36(5):862-874. https://doi.org/10.3109/07388551.2015.1051942.

Mammadov, J., Buyyarapu, R., Guttikonda, S.K., Parliament, K., Abdurakhmonov, I. Y., & Kumpatla, S. P. Wild relatives of maize, rice, cotton, and soybean: treasure troves for tolerance to biotic and abiotic stresses. Front. Plant Sci. 2018; 9:1-21.

Ma, Y., Min, L., Wang, M., Wang, C., Zhao, Y., Li, Y., Fang, Q., Wu, Y., Xie, S., Ding, Y., Su, X., Hu, Q., Zhang, Q., Li, X., & Zhang, X. 2018a. Disrupted genome methylation in response to high temperature has distinct affects on microspore abortion and anther indehiscence. The Plant Cell. 30(7):1387-1403.

Ma, Z., He, S., Wang, X., Sun, J., Zhang, Y., Zhang, G, Wu, L., Li, Z., Liu, Z., Sun, G., Yan, Y., Jia, Y., Yang, J., Pan, Z., Gu, Q., Li, X., Sun, Z., Dai, P., Liu, Z., Gong, W., Wu, J., Wang, M., Liu, H., Feng, K., & Du, X. 2018b. Resequencing a core collection of upland cotton identifies genomic variation and loci influencing fiber quality and yield. Nature genetics. 50(6): 803-813.

Majeed, S., Rana, I. A., Atif, R. M., Zulfiqar, A., Hinze, L., & Azhar, M. T. 2019a. Role of SNPs in determining QTLs for major traits in cotton. Journal of Cotton Research. 2(5):1-13. https://doi.org/10.1186/s42397-019-0022-5.

Majeed, S., Malik, T. A., Rana, I. A., & Azhar, M. T. 2019b. Antioxidant and physiological responses of upland cotton accessions grown under high-temperature regimes. Iranian Journal of Science and Technology, Transaction A, Science. 43:2759-2768. https://doi.org/10.1007/s40995-019-00781-7.

Mubarik, M. S., Ma, C., Majeed, S., Du, X., & Azhar, M. T. 2020. Revamping of cotton breeding programs for efficient use of genetic resources under changing climate. Agronomy. 10(8):1-11. https://doi.org/10.3390/agronomy10081190.

Majeed, S., Rana, I. A., Mubarik, M. S., Atif, R. M., Yang, S. H., Chung, G., Jia, Y., Du, X., Hinze, L., & Azhar, M. T. 2021. Heat stress in cotton: A review on predicted and unpredicted growth-yield anomalies and mitigating breeding strategies. Agronomy. 11(9):18-25. https://doi.org/10.3390/agronomy11091825.

Ma, Y., Min, L., Wang, J., Li, Y., Wu, Y., Hu, Q., Ding, Y., Wang, M., Liang, Y., Gong, Z., Xie, S., Su, X., Wang, C., Zhao, Y., Fang, Q., Li, Y., Chi, H., Chen, M., Khan, A. H., Lindsey, K., Zhu, L., Li, X., & Zhang X. 2021. A combination of genome-wide and transcriptome-wide association studies reveals genetic elements leading to male sterility during high temperature stress in cotton. New Phytologist. 231(1):165-181.

Manan, A., Zafar, M. M., Ren, M., Khurshid, M., Sahar, A., Rehman, A., Firdous, H., Youlu, Y., Razzaq, A., & Shakeel, A. 2022. Genetic analysis of biochemical, fiber yield and quality traits of upland cotton under high-temperature. Plant Production Science. 25(1):105-119. https://doi.org/10.1080/1343943X.2021.1972013.

Manav, N., Jit, B. P., Kataria, B., & Sharma, A. 2024. Cellular and epigenetic perspective of protein stability and its implications in the biological system. Epigenomics. 16(11-12): 879-900.

Parkash, V., Snider, J., Awori, K. J., Katta, J. R., Pilon, C., & Tishchenko, V. 2024. Distinguishing high daytime from nighttime temperature effects during early vegetative growth in cotton. Journal of Agronomy and Crop Science. 210(5): 1-14.

Pauli, D., Andrade-Sanchez, P., Carmo-Silva, A. E., Gazave, E., French, A. N., Heun, J., Hunsaker, D. J., Lipka, A. E., Setter, T. L., Strand, R. J., Thorp, K. R., Wang, S., White, J. W., & Gore, M. A. 2016. Field-based high-throughput plant phenotyping reveals the temporal patterns of quantitative trait loci associated with stress-responsive traits in cotton. G3: Genes, Genomes, Genetics. 6(4):865-879.

Qamer, Z., Chaudhary, M. T., Du, X., Hinze, L., & Azhar, M. T. 2021. Review of oxidative stress and antioxidative defense mechanisms in Gossypium hirsutum L. in response to extreme abiotic conditions. Journal of Cotton Research. 4(1):1-9.

Qin, L., Zhang, H., Li, J., Zhu, Y., Jiao, G., Wang, C., & Wu, S. 2022. Down-regulation of GhADF1 in cotton (Gossypium hirsutum) improves plant drought tolerance and increases fiber yield. The Crop Journal. 10(4): 1037-1048.

Rahman, H. U., Malik, S. A., & Saleem, M. 2004. Heat tolerance of upland cotton during the fruiting stage evaluated using cellular membrane thermostability. Field Crops Research. 85(2-3):149-158.

Rehman, A., Atif, R. M., Azhar, M. T., Peng, Z., Li, H., Qin, G, Gia, Y., Pan, Z., He, S., Qayyum, A., & Du, X. 2021. Genome wide identification, classification and functional characterization of heat shock transcription factors in cultivated and ancestral cottons (Gossypium spp.). International Journal of Biological Macromolecules. 182: 1507-1527.

Rani, S., Baber, M., Naqqash, T., & Malik, S. A. 2022. Identification and genetic mapping of potential QTLs conferring heat tolerance in cotton (Gossypium hirsutum L.) by using micro satellite marker’s approach. Agronomy. 12(6):1-21.

Ravi, S., Sharma, A., & Sharma, A. K. 2025. Roles of methyl-CpG-binding-domain (MBD) protiens in regulation of biological processes in plants. Physiology and Molecular Biology of Plants. 31: 163-171.

Saranga, Y., Jiang, C. X., Wright, R. J., Yakir, D., & Paterson, A. H. 2004. Genetic dissection of cotton physiological responses to arid conditions and their inter-relationships with productivity. Plant, Cell & Environment. 27:263-277.

Singh, R., Singh, S., Parihar, P., Mishra, R. K., Tripathi, D. K., Singh, V. P., Chauhan, D. K., & Prasad, S. M. 2016. Reactive oxygen species (ROS): Beneficial companions of plants’ developmental processes. Front. Plant Sci. 7(1299):1-19.

Su, J., Pang, C., Wei, H., Li, L., Liang, B., Wang, C., Song, M., Wang, H., Zhao, S., & Jia, X. 2016. Identification of favorable SNP alleles and candidate genes for traits related to early maturity via GWAS in upland cotton. BMC Genomics. 17(687): 1-16. https://doi.org/10.1186/s12864-016-2875-z.

Sable, A., Rai, K. M., Choudhary, A., Yadav, V. K., Agarwal, S. K., & Sawant, S. V. 2018. Inhibition of heat shock proteins HSP90 and HSP70 induce oxidative stress, suppressing cotton fiber development. Scientific Reports. 8(1):1-7. https://doi.org/10.1038/s41598-018-21866-0.

Saleem, M. F., Kamal, M. A., Anjum, S. A., Shahid, M., Raza, M. A. S., & Awais, M. 2018. Improving the performance of Bt-cotton under heat stress by foliar application of selenium. Journal of Plant Nutrition. 41(13):1711-1723. https://doi.org/10.1080/01904167.2018.1459694.

Sarwar, M., Saleem, M. F., Ullah, U., Rizwan, M., Ali, S., & Shahid, M. R. 2018. Exogenously applied growth regulators protect the cotton crop from heat induced injury by modulating plant defense mechanism. Scientific Reports. 8(1):1-5. https://doi.org/10.1038/s41598-018-35420-5.

Singh, K., Wijewardana ,C., Gajanayake, B., Lokhande, S., Wallace, T., Jones, D., & Reddy, K. R. 2018. Genotypic variability among cotton cultivars for heat and drought tolerance using reproductive and physiological traits. Euphytica. 214(57):1-22. https://doi.org/10.1007/s10681-018-2135-1.

Sadok, W., Lopez, J. R., & Smith, K. P. 2021. Transpiration increases under high‐temperature stress: Potential mechanisms, trade‐offs and prospects for crop resilience in a warming world. Plant Cell & Environment. 44(7):2102-2116. https://doi.org/10.1111/pce.13970.

Saleem, M. A., Malik, W., Qayyum, A., Ul-Allah, S., Ahmad, M. Q., Afzal, H, Amjid, M. W., Ateeq, M. F., & Zia, Z. U. 2021. Impact of heat stress responsive factors on growth and physiology of cotton (Gossypium hirsutum L.). Molecular biology reports. 48:1069-1079.

Shaheen, M., Ali, M. Y., Muhammad, T., Qayyum, M. A., Atta, S., Bashir, S, Bashir, M. A., Hashim, S., Hashem, M., & Alamri, S. 2021. New promising high yielding cotton Bt-Variety RH-647 adapted for specific agro-climatic zone. Saudi Journal of Biological Sciences. 28(8):4329-4333.

Sivakumar, S., Kumar, G. P., Vinoth, S., Siva, G., Vigneswaran, M., Gurusaravanan, P., Kanakachari, M., Kumar, T. S., Baskaran, P., & Jayabalan, N. 2021. Temporal expression profiling of GhNAC transcription factor genes in cotton cultivars under abiotic stresses. Plant Gene. 28: 100334.

Saini, D. K., Impa, S. M., McCallister, D., Patil, G. B., Abidi, N., Ritchie, G., Jaconis, S. Y., & Jagadish, K. S. V. 2023. High day and night temperatures impact on cotton yield and quality-current status and future research direction. Journal of Cotton Research. 6(1):1-17.

Sarwar, G., Abbas, H. G., Rizwan, M., Ilahi, F., Farooq, A., Farooq, J., Younas, A., Sarwar, M. K. S., Ahmad, K., & Ali, A. 2023. FH-444: A high yielding and superior fiber quality upland cotton variety suitable for cotton growin areas of punjab, Pakistan. Journal of Agricultural Research. 61(2):93-99.

Shifa, S. S., Kanok, M. M. H., Haque, M. S., Sultan, T., Pritha, K. F., Mubasshira, Al Yeamin, M., & Dipta, S. D. 2024. Influence of heat treatment and water absorption on mechanical properties of cotton-glass fiber reinforced epoxy hybrid composites: an eco-friendly approach for industrial materials. Hybrid Advances. 5: 1-9.

Su, J., Li, D., Yuan, W., Li, Y., Ju, J., Wang, N, Ling, P., Feng, K., & Wang, C. 2024. Integrating RTM-GWAS and meta QTL data revealed genomic regions and candidate genes associated with the first fruit branch node and its height in upland cotton. Theoretical and Applied Genetics. 137(207).

Tariq, M., Yasmeen, A., Ahmad, S., Hussain, N., Afzal, M. N., & Hasanuzzaman, M. 2017. Shedding of fruiting structures in cotton: Factors, compensation and prevention. Tropical and Subtropical Agroecosystems. 20(2):251-262. https://doi.org/10.56369/tsaes.2286.

Tibbs Cortes, L., Zhang, Z., & Yu, J. 2021. Status and prospects of genome‐wide association studies in plants. The plant genome. 14(1): 1-17.

Tian, Y., Liu, P., Kong, D., Nie, Y., Xu, H., Han, X., Sang, W., & Li, W. 2025. Genome-wide association analysis and KASP markers development for protein quality traits in winter wheat. BMC Plant Biology. 25(149): 1-17.

Wang, Q., Wang, J., Huang, X., Liu, Z., Jin, W., Hu, W., Meng, Y., & Zhou, Z. 2024a. Phosphorus application under continuous wheat-cotton straw retention enhanced cotton root productivity and seedcotton yield by improving the carbohydrate metabolism of root. Field Crops Research. 317, 109541.

Wang, D., Lin, H., Shan, Y., Song, J., Zhang, D. D., Dai, X. F., Han, D., & Chen, J. Y. 2024b. The potential of Burkholderia gladioli KRS027 in plant growth promotion and biocontrol against Verticillium dahliae revealed by dual transcriptome of pathogen and host. Microbiological Research. 287: 1-14.

Xiao, Q., Bai, X., Zhang, C., & He, Y. 2022. Advanced high-throughput plant phenotyping techniques for genome-wide association studies: A review. Journal of advanced research. 35: 215-230.

Yasin, M., Ali, A., Shaheen, M., Khaliq, A., Ahmad, S., Rizwan, M, H. G. Abbas, & Ali, Q. 2019. Characterization of yield potential for RH-662: A new high yielding, stress and salinity tolerant cotton variety. International Journal of Botany Studies. 4(2): 66-71.

Yasin, M., Ali, A., Masood, S. A., Anum, W., Ali, L., Ali, B, Khan, A. M., & Ali, Q. 2020. Evaluation of release of new cotton variety “RH-668”. International Journal of Biology, Pharmacy and Allied Sciences. 9(2):270-281.

Yasir, M., Kanwal, H. H., Hussain, Q., Riaz, M. W., Sajjad, M., Rong, J., & Jiang, Y. 2022. Status and prospects of genome-wide association studies in cotton. Frontiers in Plant Science. 13:1-18.

Yousaf, M. I., Hussain, Q., Alwahibi, M. S., Aslam, M. Z., Khalid, M. Z., Hussain, S., Zafar, A., Shah, S. A. S., Abbasi, A. M., Mehboob, A., Riaz, M. W., & Elshikh, M. S. 2023. Impact of heat stress on agro-morphological, physio-chemical and fiber related parameters in upland cotton (Gossypium hirsutum L.) genotypes. Journal of King Saud University-Science. 35(1):1-8.

Zahid, K. R., Ali, F., Shah, F., Younas, M., Shah, T., Shahwar, D., Hassan, W., Ahmad, Z., Qi, C., Lu, Y., Iqbal, A., & Wu W. 2016. Response and tolerance mechanism of cotton Gossypium hirsutum L. to elevated temperature stress: A review. Frontiers in Plant Science. 7:1-13.

Zhai, N., Jia, H., Liu, D., Liu, S., Ma, M., Guo, X., & Li., H. 2017. GhMAP3K65, a cotton Raf-like MAP3K gene, enhances susceptibility to pathogen infection and heat stress by negatively modulating growth and development in transgenic Nicotiana benthamiana. International journal of molecular sciences. 18(11): 1-22.

Zhang, M., Zhang, X., Guo, L., Qi, T., Liu, G., Feng, J., Shahzad, K., Zhang, B., Li, X., Wang, H., Tang, H., Qiao, X., Wu, J., & Xing, C. 2020. Single-base resolution methylome of cotton cytoplasmic male sterility system reveals epigenomic changes in response to high-temperature stress during another development. Journal of Experimental Botany. 71(3):951-969.

Zhang, L., Tian, W., Huang, G., Liu, B., Wang, A., Zhu, J., & Guo, X. 2021. The SikCuZnSOD3 gene improves abiotic stress resistance in transgenic cotton. Molecular Breeding. 41(26): 1-17.

Zhang, J., Loka, D. A., Wang, J., Ran, Y., Shao, C., Tuersun, G., Li, Y., Wang, S., Zhou, Z., & Hu, W. 2024. Co-occurring elevated temperature and drought stress inhibit cotton pollen fertility by disturbing anther carbohydrate and energy metabolism. Industrial Crops and Products. 208: 117894.

Zafar, M. M., Jia, X., Shakeel, A., Sarfraz, Z., Manan, A., Imran, A., Mo, H., Ali, A., Youlu, Y., Razzaq, A., Iqbal, M. S., & Ren, M. 2022. Unraveling heat tolerance in upland cotton (Gossypium hirsutum L.) using univariate and multivariate analysis. Frontiers in Plant Science. 12:1-17.

Downloads

Published

2025-08-31

Issue

Section

Review Articles

How to Cite

Impact of heat stress, molecular responses, and breeding strategies for heat tolerance in cotton (Gossypium hirsutum L.). (2025). Zoo Botanica, 3(2), 435-453. https://doi.org/10.55627/zoobotanica.003.02.1197

Similar Articles

11-20 of 53

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)