Optimization of microwave-assisted extraction (MAE) protocol and study of antimicrobial activity of Bergenia ciliata (Haw.) Sternb.
DOI:
https://doi.org/10.55627/zoobotanica.003.02.1452Keywords:
Antibacterial, antifungal, Bergenia ciliate, microwave-assisted extraction, MAE, phenolic compoundsAbstract
Bergenia ciliata is an effective medicinal plant since it contains a significant number of bioactive chemicals and has a broad spectrum of biological action. The current study was conducted to evaluate the antimicrobial activity of Bergenia ciliata rhizome extract using microwave-assisted extraction (MAE) with 1 g of plant material in 10 mL of solvent ratio. For extraction, several factors such as power level, time, ethanol and petroleum ether solvents were used. The highest extract weight of 0.957 g was discovered in ethanol using a combination of 200 W (power) and 150 seconds (time). The phenolic content in the rhizome of Bergenia ciliata was also determined by measuring absorption at 765 nm with a UV spectrophotometer. The highest total phenolic content in Bergenia ciliata rhizomes extract with ethanol was reported to be 62.43063a ± 0.1 mg/g at a power level of 200 W after 150 seconds of microwave irradiation. Antimicrobial activity was tested using selected bacterial (Escherichia coli, Salmonella enterica and Staphylococcus aureus) and fungal (Trichoderma harzianum, Alternaria alternata, and Fusarium oxysporum) strains after extraction. Ethanol showed the highest antimicrobial action. By utilizing the anti-microbial properties of Bergenia ciliata, current research can be expanded to examine alternative drug discoveries.
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