Biological alternatives to herbicides: Unlocking the potential of bioherbicides and plant-based alternatives
DOI:
https://doi.org/10.55627/zoobotanica.003.02.1470Keywords:
Bioherbicides, eco-friendly weed management, integrated weed management, allelopathy, weed resistance management, environmental safety of herbicidesAbstract
The agricultural sector is under growing pressure to improve crop yields and production efficiency in order to meet the escalating demands for food worldwide. Weeds, crop pathogens, and insect pests remain major biotic constraints, with weeds persistently ranked among the most detrimental factors limiting crop yields. Weeds pose a persistent challenge in crop husbandry, competing aggressively with cultivated plants for essential growth resources including soil moisture, nutrients, and sunlight. Such competition reduces crop yields, besides affecting biodiversity. Conventional weed management practices rely heavily on synthetic herbicides, have raised serious concerns. These concerns include environmental contamination, the evolution of herbicide-resistant weed populations, and harmful impacts on non-target organisms such as pollinators and beneficial soil microorganisms. Therefore, there is a growing demand to explore sustainable weed management alternatives that safeguard agronomic productivity while preserving ecological balance. Conventional herbicide-based weed management has raised concerns due to environmental contamination, the emergence of herbicide-resistant weed biotypes, and detrimental impacts on non-target organisms. These challenges have accelerated the search for sustainable weed management strategies. Biological approaches, such as bioherbicides derived from fungi, bacteria, and allelopathic plants, provide environmentally friendly options consistent with the principles of sustainable agriculture. Among these, essential oils—rich in phytotoxic terpenoids—demonstrate fortified potential as natural alternatives due to their selectivity and minimal environmental impact. Biological approaches suppress weeds through natural allelopathic and microbial mechanisms, enhance agro-biodiversity, improve soil health leading to reduced reliance on synthetic herbicides. The present article underscores the potential of integrating bioherbicides and other allelopathic agents into weed management programs to boost sustainable crop production systems and strengthen ecological resilience
References
Ahmad, D., M.I. Chani and A.A. Humayon. (2017). Major crops forecasting area, production and yield evidence from agriculture sector of Pakistan. Sarhad Journal of Agriculture, 33, 385-396.
Ahmad, M., (2019). Developing a competitive agriculture and agro-based industry under CPEC. In China’s Belt and Road Initiative in a Global Context: Volume II: The China Pakistan Economic Corridor and its Implications for Business (pp. 227-269). Cham: Springer International Publishing.
Ali, I.B.E.H., Chaouachi, M., Bahri, R., Chaieb, I., Boussaïd, M. and Harzallah-Skhiri, F., (2015). Chemical composition and antioxidant, antibacterial, allelopathic and insecticidal activities of essential oil of Thymus algeriensis Boiss. et Reut. Industrial crops and products, 77, 631-639.
Almarie, A.A., Mamat, A.S. and Wahab, Z. (2016) Allelopathic potential of Cymbopogon citratus against different weed species. Indian Research Journal of Pharmaceutical Science 3,324–330
Almarie, A.A., (2022). Phytotoxic Activity of essential oils. In Essential Oils: Applications and Trends in Food Science and Technology (pp. 263-279). Cham: Springer International Publishing.
Arya, S and Pandey, M. (2024). Chapter 17 Bioherbicides for Integrated Weed Management. In Biorationals and Biopesticides; pp. 355–372. De Gruyter: Berlin, Germany
Aktar, M. W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in agriculture: Their benefits and hazards. Interdisciplinary Toxicology, 2(1), 1. https://doi.org/10.2478/v10102-009-0001-7
Asghar, W. and Kataoka, R., 2021. Effect of co-application of trichoderma spp. With organic composts on plant growth enhancement, soil enzymes and fungal community in soil. Archives of Microbiology, 203, 4281–4291. https://doi.org/10.1007/s00203-021-02413- 4
Asghar, W., Craven, K.D., Kataoka, R., Mahmood, A., Asghar, N., Raza, T., Iftikhar, F., 2024. The application of trichoderma spp., an old but new useful fungus, in sustainable soil health intensification: A comprehensive strategy for addressing challenges. Plant Stress, 100455. https://doi.org/10.1016/j.stress.2024.100455.
Bailey K.L. (2014). The bioherbicide approach to weed control using plant pathogens; pp. 245–266. Integrated Pest Management. Academic Press; Cambridge, MA, USA.
Bashar, H.K., Juraimi, A.S., Ahmad-Hamdani, M.S., Uddin, M.K., Asib, N., Anwar, M.P., Rahaman, F., Karim, S.R., Haque, M.A., Berahim, Z. and Nik Mustapha, N.A., (2022). Determination and quantification of phytochemicals from the leaf extract of parthenium hysterophorus L. And their physio-biochemical responses to several crop and weed species. Plants, 11(23), p.3209.
Baz, K., Zhen, Z. and Ali, H., (2025). Impact of Advanced Agriculture Technologies and Energy Consumption on Crop Yields in Modern Agriculture Using Deep Learning Techniques. Food and Energy Security, 14, p.e70076.
Becerra-Alvarez, A., Zhang, Z. and Al-Khatib, K., (2025). Physiological action of bioherbicides in weed control: a systematic review. Frontiers in Agronomy, 7, p.1633565.
Benbrook, C., Kegley, S. and Baker, B., 2021. Organic farming lessens reliance on pesticides and promotes public health by lowering dietary risks. Agronomy, 11, 1-36.
Bordin, E.R., Frumi Camargo, A., Stefanski, F.S., Scapini, T., Bonatto, C., Zanivan, J., Preczeski, K., Modkovski, T.A., Reichert, F., Jr., Mossi, A.J. et al. (2021). Current Production of Bioherbicides: Mechanisms of Action and Technical and Scientific Challenges to Improve Food and Environmental Security. Biocatal Biotransformation, 39, 346–359
Boutagayout, A., Belmalha, S., Nassiri, L., El Alami, N., Jiang, Y., Lahlali, R. and Bouiamrine, E.H., 2024. Weed competition, land equivalent ratio and yield potential of faba bean (Vicia faba L.)-cereals (Triticum aestivum L. and/or Avena sativa L.) intercropping under low-input conditions in Meknes region, Morocco. Vegetos, 37(3), pp.866-879.
Boyette, C.D and Hoagland, R.E. (2015). Bioherbicidal potential of Xanthomonas campestris for controlling Conyza canadensis. Biocontrol Science and Technology, 25, 229-237
Chakraborty, A. and Ray, P., (2021). Mycoherbicides for the noxious meddlesome: can Colletotrichum be a budding candidate? Frontiers in Microbiology, 12, p.754048.
Cheng, L., DiTommaso, A. and Kao-Kniffin, J., (2022). Opportunities for microbiome suppression of weeds using regenerative agricultural technologies. Frontiers in Soil Science, 2, p.838595.
Cordeau, S., Triolet, M., Wayman, S., Steinberg, C. and Guillemin, J.P., (2016). Bioherbicides: Dead in the water? A review of the existing products for integrated weed management. Crop protection, 87, 44-49.
Crop Protection Chemicals Global Market Report 2025. https://www.thebusinessrese archcomp
Dayan, F.E., Cantrell, C.L. and Duke, S.O., (2009). Natural products in crop protection. Bioorganic & medicinal chemistry, 17, 4022-4034.
Dixit, S., Sivalingam, P.N., Baskaran, R.M., Senthil-Kumar, M. and Ghosh, P.K., (2024). Plant responses to concurrent abiotic and biotic stress: unravelling physiological and morphological mechanisms. Plant Physiology Reports, 29, 6-17. DOI | http://dx.doi.org/10.17582/journal.sja/2017/33.3.385.396
Duke, S.O., (2024). Why are there no widely successful microbial bioherbicides for weed management in crops?. Pest Management Science, 80, 56-64.
Duke, S.O., Pan, Z., Bajsa-Hirschel, J. and Boyette, C.D., (2022). The potential future roles of natural compounds and microbial bioherbicides in weed management in crops. Advances in Weed Science, 40(spe1), p.e020210054.
El Mahdi, J., Tarraf, W., Ruta, C., Piscitelli, L., Aly, A. and De Mastro, G., 2020. Bio-herbicidal potential of the essential oils from different Rosmarinus officinalis L. chemotypes in laboratory assays. Agronomy, 10, 2-17. doi:10.3390/agronomy10060775
Fahad, S., Hussain, S., Chauhan, B.S., Saud, S., Wu, C., Hassan, S., Tanveer, M., Jan, A., Huang, J. 2015. Weed Growth and Crop Yield Loss in Wheat as Influenced by Row Spacing and Weed Emergence Times. Crop Protection, 71, 101–108.
Gharde, Y., Singh, P.K., Dubey, R.P. and Gupta, P.K., (2018). Assessment of yield and economic losses in agriculture due to weeds in India. Crop Protection, 107, 12-18.
Gill, S.S. and Tuteja, N. (2010). Reactive Oxygen Species and Antioxidant Machinery in Abiotic Stress Tolerance in Crop Plants. Plant Physiology and Biochemistry, 48, 909-930. http://dx.doi.org/10.1016/j.plaphy.2010.08.016
Gu, Q., Chu, S., Huang, Q., Chen, A., Li, L., & Li, R. (2023). Colletotrichum echinochloae: A potential bioherbicide agent for control of barnyardgrass (Echinochloa crus-galli (L.) Beauv.). Plants, 12(3). https://doi.org/10.3390/plants12030421
Harding, D.P. and Raizada, M.N., (2015). Controlling weeds with fungi, bacteria and viruses: a review. Frontiers in plant science, 6, p.659.
Hasan, M., Ahmad-Hamdani, M.S., Rosli, A.M. and Hamdan, H., (2021). Bioherbicides: An eco-friendly tool for sustainable weed management. Plants, 10, p.1212.
Hasan, M., Mokhtar, A.S., Mahmud, K., Berahim, Z., Rosli, A.M., Hamdan, H., Motmainna, M. and Ahmad-Hamdani, M.S., (2022). Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock. Scientific Reports, 12(1), p.19602.
He, Z., Zhang, X., Fan, S., Chen, R., Sun, J., Ma, F., Zhao, P., Liu, J., Zhang, H. and Liu, C., 2025. Effects of the interspecific competition between Eichhornia crassipes and Alternanthera philoxeroides and the additional impact of their biological control agents: relevance for aquatic weed management. Hydrobiologia, 852(8), 2523-2536.
Horvath, D.P., Clay, S.A., Swanton, C.J., Anderson, J.V. and Chao, W.S., (2023). Weed-induced crop yield loss: a new paradigm and new challenges. Trends in Plant Science, 28, 567-582.
Inderjit, Pollock J.L., Callaway, R.M., Holben, W. (2008) Phytotoxic Effects of (6)-Catechin In vitro, in Soil, and in the Field. PLoS ONE 3(7): e2536. doi:10.1371/ journal.pone.0002536
Irshad, A and Cheema, Z.A. (2004). Influence of some plant water extracts on the germination and seedling growth of barnyard grass (E. crus-galli (L) Beauv). Pakistan Journal of Science and Industrial Research, 43, 222–226.
Islam, A.M., Karim, S.M.R., Kheya, S.A. and Yeasmin, S., (2024). Unlocking the potential of bioherbicides for sustainable and environment friendly weed management. Heliyon, 10(16).
Jesudas, P.A., Kingsley, S.J. and Ignacimuthu, S., (2014). Sorgoleone from Sorghum bicolor as a potent bioherbicide. Research Journal of Recent Sciences, 3 (ISC-2013): 32-36 ref. 45
Jonker, J., and Pennink, B.W. The Essence of Methodology Pp: 21-42. The Essence of Research Methodology A Concise Guide for Master and PhD Students in Management Science. DOI 10.1007/978-3-540-71659-4 Springer Heidelberg Dordrecht London New York.
Juroszek, P. and von Tiedemann, A.V., (2013). Plant pathogens, insect pests and weeds in a changing global climate: a review of approaches, challenges, research gaps, key studies and concepts. The Journal of Agricultural Science, 151, 163-188.
Kavya, D. and Nisarga, G. D., (2025). Chapter 5 Sustainable Weed Management: Strategies and Innovation. Practices and Innovations, in Agronomy for Sustainable Agriculture: Practices and Innovations (Edited by : Dr. Mayur K. Rathwa, Th. Nengparmoi, Dr. Swati Kadam, Chunchu Suchith Kumar and Dr. Ch V L Kishore) Pp.84-113.
Khamare, Y., Chen, J. and Marble, S.C., (2022). Allelopathy and its application as a weed management tool: A review. Frontiers in Plant Science, 13, p.1034649.
Khan, B.A., Alawadi, H.F.N., Nadeem, M.A., Liaquat, G., Mahmood, A., Qamar, R., Nazim, M., Ayub, H. and Abbas, T., (2025). Chapter 13. Biological control of weeds, using bioherbicides, nematodes, insect and fungal macromolecules. In, Fungal Macromolecule Applications in Life Sciences: Biological Activity and Medical, Industrial, and Agricultural Applications. 301-326. DOI: https://doi.org/10.1016/B978-0-443-30102-5.00013-2
Khan, I., Akram, A., Fatima, S., Ahmad, B., Rehman, Z., Arshad, N., Sattar, A. and Ahmad, Z., (2022). Problems of agriculture in Pakistan: an insight into their solution. Pakistan Journal of Biotechnology, 19, 73-83.
Liu, S., S., Cheng, S., Jia, J. and Cui, J., (2022). Resource efficiency and environmental impact of juglone in Pericarpium Juglandis: A review. Frontiers in Environmental Science, 10, p.999059.
Marrone, P.G., (2024). Status of the biopesticide market and prospects for new bioherbicides. Pest Management Science, 80, 81-86.
Mehrabi, Z., (2023). Likely decline in the number of farms globally by the middle of the century. Nature Sustainability, 6, 949-954.
Merfield, C.N., (2025). Weeds as a social construct. In Agroecology of Edible Weeds and Noncrop Plants (pp. 119-131). Academic Press.
Mugford, S.T. and Osbourn, A., (2012). Saponin synthesis and function. In Isoprenoid synthesis in plants and microorganisms: New concepts and experimental approaches (pp. 405-424). New York, NY: Springer New York.
Nath, C.P., Singh, R.G., Choudhary, V.K., Datta, D., Nandan, R. and Singh, S.S., (2024). Challenges and alternatives of herbicide-based weed management. Agronomy, 14(1):126.
Neal, J.C., (2024). Biological control of weeds in turfgrass: opportunities and misconceptions. Pest Management Science, 80(1):40-48.
Nayak, K., Tiwari, R., Parte, V., Chouhan, M., Kumar, P., Verma, B., Jayant, R. and Pandey, A., 2024. Bio-herbicides: An eco-friendly approach for integrated weed management. International Journal of Advanced Biochemistry Research, 8,828-840
Pacanoski, Z., (2015). Bioherbicides. Herbicides, Physiology of Action, and Safety. InTech. Available at: http://dx.doi.org/10.5772/61528.
Pathak, H., Nayak, A.K., Maiti, D., Kumar, G.A.K., Reddy, J.N., Rath, P.C., Swain, P. and Bhagawati, R., (2019). Design and layout.
Pedroso, R.M. and Moretti, M.L., (2025). Weed Resistance to Herbicides and Genetically Engineered, Herbicide-Resistant Soybeans as a Tool for Weed Control: Past, Present, and Future of Adoption. In Soybean Production Technology: Crop Pests and Diseases (pp. 327-351). Singapore: Springer Nature Singapore.
Petersen, J., Belz, R., Walker, F. and Hurle, K., (2001). Weed suppression by release of isothiocyanates from turnip‐rape mulch. Agronomy Journal, 93, 37-43.
Puig, C.G., Revilla, P., Barreal, M.E., Reigosa, M.J. and Pedrol, N., (2019). On the suitability of Eucalyptus globulus green manure for field weed control. Crop Protection, 121, 57-65.
Radhakrishnan, R., A.A. Alqarawi, E.F. Abd-Allah, (2018). Bioherbicides: current knowledge on weed control mechanism, Ecotoxicol. Environ. Safe. 158, 131–138. https://doi.org/10.1007/s10526-018-9890-8
Raju, G.V., (2025). Weeds and their Management. Science and Sustainability, In M Agriculture Science and Sustainability Edited By: Preeti lata Singh, Abhay Dubey, Akhil Bharti, Madhu Devi and Ravinder Kaur. p.165.
Rakshit, A., Meena, V.S., Abhilash, P.C., Sarma, B.K., Singh, H.B., Fraceto, L., Parihar, M. and Singh, A.K. eds., (2021). Biopesticides: Volume 2: Advances in bio-inoculants. Woodhead Publishing.
Raza, T., Qadir, M.F., Imran, S., Khatoon, Z., Khan, M.Y., Mechri, M., Asghar, W., Rehmani, M.I.A., de los Santos Villalobos, S., Mumtaz, T. and Iqbal, R., (2025). Bioherbicides: revolutionizing weed management for sustainable agriculture in the era of One-health. Current Research in Microbial Sciences, p.100394.
Roberts, J., Florentine, S., Fernando, W.D. and Tennakoon, K.U., (2022). Achievements, developments and future challenges in the field of bioherbicides for weed control: A global review. Plants, 11, 2242.
Schwarzländer, M., Hinz, H.L., Winston, R.L. and Day, M.D., (2018). Biological control of weeds: an analysis of introductions, rates of establishment and estimates of success, worldwide. BioControl, 63, 319-331.
Schütte G, Eckerstorfer M, Rastelli V, Reichenbecher W, Restrepo-Vassalli S, Ruohonen-Lehto M, Saucy AW, Mertens M. Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants. Environmental Sciences Europe 2017; 29(1):5. doi: 10.1186/s12302-016-0100-y. Epub 2017 Jan 21. PMID: 28163993; PMCID: PMC5250645.
Sherwani, S. I., Arif, I. A., & Khan, H. A. (2015). Modes of Action of Different Classes of Herbicides. InTech. Doi: 10.5772/61779
Shrestha, A., Anwar, M.P., Islam, A.K.M.M., Gurung,T., Dhakal, S., Tanveer, A. Javaid, M., Nadeem, M. M., Ikram, N. A. (2021). Weed science as a new discipline and its status in some south Asian universities and colleges: examples from Bangladesh, Bhutan, Nepal, and Pakistan, CAB Rev. 16, 1–14, https://doi.org/ 10.1079/PAVSNNR202116017
Sims, B., Corsi, S., Gbehounou, G., Kienzle, J., Taguchi, M. and Friedrich, T., 2018. Sustainable weed management for conservation agriculture: Options for smallholder farmers. Agriculture, 8(8), p.118. https://doi.org/10.3390/agriculture8080118
Size, K.M., 2024. Share & Trends Report, 2022–2030 [online]
Soltys, D., Krasuska, U., Bogatek, R. and Gniazdowska, A., (2013). Allelochemicals as bioherbicides—Present and perspectives. In Herbicides-Current research and case studies in use. IntechOpen.
Srimathi, K., (2024). Bio Herbicide. Sustainable Agriculture in the 21st Century, p.28.
Stefanski, F.S., Camargo, A.F., Scapini, T., Bonatto, C., Venturin, B., Weirich, S.N., Ulkovski, C., Carezia, C., Ulrich, A., Michelon, W. and Soares, H.M., (2020). Potential use of biological herbicides in a circular economy context: a sustainable approach. Frontiers in Sustainable Food Systems, 4, p.521102.
Subramanian, K.S., 2025. Green Herbicide Revolution: Development and Characterization of Eucalyptus globulus Essential Oil-Loaded Nanoemulsion for Sustainable Weed Control. BioNanoScience, 15, 1-15.
Thiour-Mauprivez, C., Martin-Laurent, F., Calvayrac, C. and Barthelmebs, L., 2019. Effects of herbicide on non-target microorganisms: towards a new class of biomarkers? Science of the Total Environment, 684, 314-325
Triolet, M., Guillemin, J.P., Andre, O. and Steinberg, C., (2020). Fungal‐based bioherbicides for weed control: a myth or a reality?. Weed Research, 60, 60-77.
Ulas, F., Torun, H., Beffa, R. and Mennan, H., (2025). Global trends and research ınsights on herbicide resistance: a bibliometric analysis. Phytoparasitica, 53, 1-19.
Varah A, Ahodo K, Shaun RC, Hicks HL, Comont D, Crook L, Hull R, Neve P, Childs DZ, Freckleton RP, Norris K (2019) The costs of human-induced evolution in an agricultural system. Nature Sustainability. 3:63-71.
Verdeguer, M., Blázquez, M.A. and Boira, H., (2012). Chemical composition and herbicidal activity of the essential oil from a Cistus ladanifer L. population from Spain. Natural Product Research, 26, 1602-1609.
Villavicencio-Vásquez, M., Espinoza-Lozano, F., Espinoza-Lozano, L. and Coronel-León, J., (2025). Biological control agents: mechanisms of action, selection, formulation and challenges in agriculture. Frontiers in Agronomy, 7, p.1578915.
Wesołowska, A., Jadczak, P., Kulpa, D. and Przewodowski, W., 2019. Gas chromatography-mass spectrometry (GC-MS) analysis of essential oils from AgNPs and AuNPs elicited Lavandula angustifolia in vitro cultures. Molecules, 24(3), p. 606. https://doi.org/10.3390/molecules24030606
Weston, L.A., Alsaadawi, I.S. and Baerson, S.R., (2013). Sorghum allelopathy—from ecosystem to molecule. Journal of Chemical Ecology, 39,142-153.
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