Optimized In-Vitro Regeneration of Peanut (Arachis hypogaea L.) via Callus Induction and Embryogenesis
DOI:
https://doi.org/10.55627/agrivet.004.01.01172Keywords:
Arachis hypogaea L, Embryogenesis and Callus, Auxin (NAA) and Cytokinin (BA)Abstract
To investigate the effect of the growth regulators of auxin plants (NAA) and cytokinin (BAP) on the regeneration potential of two varieties of Bard-479 and Potohar peanuts in-vitro culture. As explants, 22-day-old seedlings derived from callus and the embryonic axis was used. The explant as cotyledonary nodes were cultured in basal medium (MS) of medium resistance supplemented with different combinations and concentrations of auxin (NAA) and cytokinin (BAP) for the primary proliferation of the shoots. For the culture of embryos, Picloram (19mg/l), BAP (0.1, 0.2, 0.3, 0.4 mg / l) in combination with NAA (0.1, 0.2, 0.5mg/l) and 2, 4 - D (0.1-0.3 mg/l) together with BAP and NAA used with the same combinations and concentrations were used for callus. The whole plant's shoots, leaves, and regeneration were scored after four weeks according to the percentage of explants that produced shoots, calluses, embryos, and roots per explants. The best shoots were observed at 0.4 mg / l BAP with 0.1 mg / l NAA; 87% were observed in BARD-479, and POTOHAR showed 75% regeneration by embryogenesis. Callus showed 73% recovery in BARD-479 and 65%in POTOHAR. Similarly, some media could produce structures similar to the roots at less than 10% frequency. For Bard-479, the best rate for roots were found with 0.3 mg / l IBA and 0.2 mg / l NAA, i.e. (95%), and for Potohar (87%) is recorded. ANOVA (analysis of variance) reported in Table 1-8 showed that callus and embryogenesis were of great importance.
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