Integrated Management of Tikka Disease in Groundnut Using Chemical, Botanical, and Biocontrol Approaches
DOI:
https://doi.org/10.55627/agrivet.004.03.01520Keywords:
Groundnut, Cercospora arachidicola, Cercosporidium personatum, Mancozeb, Trichoderma, Botanicals, BiocontrolAbstract
Groundnut (Arachis hypogaea) in Pakistan faces a major challenge from tikka disease, caused by Cercospora arachidicola and Cercosporidium personatum. The present study was undertaken to isolate the causal agents and evaluate the efficacy of fungicides and Bio-botanical protectants. Infected leaf samples were collected from the University Research Farm, Koont (Chakwal), and pathogens were isolated based upon cultural and morphological characteristics (e.g., colony color, texture, spore size and shape). Pathogenicity was confirmed by fulfilling Koch’s postulates proving Cercospora arachidicola and Cercosporidium personatum to be the actual cause for tikka disease of ground nut. Following isolation, pot experiment was conducted under Completely Randomized Design (CRD) comprising nine treatments and three replications. Means were analysed statistically by applying ANOVA using Statstix 8.1 software. Varying concentrations of Mancozeb (0.1% and 0.2%), neem extract (5% and 10%), garlic extract (5% and 10%), and Trichoderma spp. (5 mL and 10 mL), with combined treatments of Mancozeb 0.2%, neem 10%, garlic 10%, and Trichoderma 10 mL. Key parameters viz., disease incidence, severity, plant height, number of leaves, fresh and dry biomass were recorded. The results revealed combined treatments effectively reducing disease incidence by 48.66% and severity by 50.59% and enhancing plant height (30.32 cm), leaf number (15), and biomass. The disease was supressed highly using 0.2% Mancozeb, followed by moderate Trichoderma treatments while neem and garlic extracts showed minimum potential. The study highlighted combined biogenic inputs to manage tikka disease and improve plant growth with effectivity, sustainability, and eco-friendly strategies.Keywords: Groundnut, Cercospora arachidicola, Cercosporidium personatum, Mancozeb, Trichoderma, Botanicals, Biocontrol.
References
Akinbode, OA (2010). Evaluation of antifungal efficacy of some plant extracts on Curvularia lunata, the causal organism of maize leaf spot. African Journal of Environmental Science and Technology 4(11):797 800.
Azmat, M.A., Khan, A.A., Saeed, A., Ashraf, M. and Niaz, S., 2012. Screening pea germplasm against Erysiphepolygoni for disease severity and latent period. International Journal of Vegetable Science, 18(2), pp.153-160.
Balasubramanian, P., Subbulakshmi, B., Balmurugan, M., Gurumeenakshi, G., Prasanth, R.C., Deepika, R. and Surya, R., 2024. Nutritional profiling and its significance in groundnut: A Review. Asian Journal of Dairy & Food Research, 43(4).
Chen, A., D.P. Le, L.J. Smith, D. Kafle, E.A.B. Aitken and D.M. Gardiner. 2024. Assessing the Pathogenicity of Berkeleyomyces rouxiae and Fusarium oxysporum f. sp. vasinfectum on Cotton (Gossypium hirsutum) Using a Rapid and Robust Seedling Screening Method. JoF. 10:715.
Chen, X., Lu, Y., Liu, X., Gu, Y., & Li, F. (2025). Trichoderma: Dual Roles in Biocontrol and Plant Growth Promotion. Microorganisms, 13(8), 1840.
Crop Reporting Service, Agriculture Department, Punjab. Final Estimate of Groundnut Crop Punjab 2022–23. https://crs-agripunjab.punjab.gov.pk/system/files/GroundNut%20Final%202022-23.pdf
Devi, P. A. (2015). Evaluation of new fungicide product (Carbendazim 12%+ Mancozeb 63% WG) for its efficacy against groundnut diseases.
Dutta, P., Deb, L., Gogoi, J., Mahanta, M., Kumari, A., Yasin, A. and Sharma, A., 2021. UmTricho” a liquid bioformulation of indigenous strain of Trichoderma harzianum effectively managed the tikka disease (Cercospora spp.) of groundnut, Arachis hypogea l. under the agroecological condition of meghalaya. In Biol. Forum (Vol. 13, No. 2, pp. 529-535).
Hussain, M., Ali, S., Zeeshan, M., Nabi, G., Saadia, Muzaffar, W., Saeed, M. and Ahmad, B., 2022. Attock-2019: a high-yielding and disease-tolerant peanut (Arachis hypogaea L.) cultivar for Barani tract of Punjab, Pakistan. 54(4), 733-741.
Kumar, S., Jaiswal, S., Lal, A.A., Kumar, A. and Verma, A., 2017. Influenced of natural products and bio-fungicide against tikka disease of groundnut caused by Cercospora spp. The Pharma Innovation, 6(3), 213.
Lee, S. M., Park, J. H., & Kim, Y. C. (2019). Biological control of soil-borne pathogens by Trichoderma spp. Mechanisms and applications. Journal of Mycology, 49(3), 201-210.
Mapari, A. R., & Zacharia, S. (2017). Efficacy of bio agents and botanicals against leaf spot (Cercospora arachidicola Hori) of groundnut (Arachis hypogaea L.). Journal of Pharmacognosy and Phytochemistry,6(5), 504-506.
Moosa, A., Farzand, A., Sahi, S.T., Khan, S.A., Abbas, M.F., Abbas, A. and Binyamin, R., 2020. First report of post-harvest Fusarium rot caused by Fusarium oxysporum on Citrus reticulata Blanco cv. ‘Kinnow’ in Pakistan. Journal of Plant Pathology, 102(3), 945-946.
Nigam, S. N. (2014). Groundnut at a glance. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
Niwas, R., Chand, G., Vishwakarma, R. and Maurya, S., 2021. Cultural Practices in Sustainable Plant Disease Management: The Innovative Approaches.
Smith, D., Brown, A., & Wang, L. (2020). Comparative analysis of synthetic and biocontrol agents in the management of foliar fungal diseases. Crop Protection, 134, 105147.
Srinivas, B., S.A. Basha, B.V. Sagar, C.V.S. Kumar and G.K. Reddy. 2024. Isolation, Purification and Pathogenicity Assessment of Fusarium oxysporum. f.sp. ciceris Inciting wilt disease in chickpea. IJEP. 11:153–159.
Sundaramoorthy, S., & Ivin, J. J. S. (2023). Molecular Variability in Phaeoisariopsis personata Causing Late Tikka Leaf Spot of Ground Nut in Cuddalore District of Tamil Nadu. Plant Cell Biotechnology and Molecular Biology, 23-29.
Syamsia, S., A. Idhan, H. Latifah, N. Noerfityani and A. Akbar. 2021. Alternative medium for the growth of endophytic fungi. IOP Conf. Ser.: Earth Environ. Sci. 886:012045.
Trading economics. (2023). Pakistan Imports of peanuts (ground-nuts), raw from Nigeria
Zamurrad, M., Tariq, M., Shah, F.H., Subhani, A., Ijaz, M., Iqbal, M.S. and Koukab, M., 2013. Performance based evaluation of groundnut genotypes under medium rainfall conditions of Chakwal. Journal of Agri-Food and Applied Sciences, 1(1), 9-12.
Zeng, Y., Zhao, L., & Ma, X. (2019). Antifungal activity of garlic extract and its potential as a natural pesticide. Journal of Plant Diseases and Protection, 126(6), 545–552.
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Copyright (c) 2025 Muhammad Nawaz, M. Inam ul Haq, Syed Zulfiqar Ali, Gulshan Irshad, Atta ul Rehman, Mehak Zahra, Hurmain Aslam, Laiba Zarar Noor, Muhammad Naveed Tahir
This work is licensed under a Creative Commons Attribution 4.0 International License.
